Technical Info
Tips for Electrophoresis
- Basic operation of SDS-PAGE (Polyacrylamide Gel Electrophoresis)
- Method for extracting protein from tissue or cell
- Sample preparation for electrophoresis
- Preparation of polyacrylamide gel
- Polyacrylamide gel electrophoresis (PAGE)
- Principles and method of the 2-dimensional electrophoresis
- Protein electrophoresis gel staining
- Agarose gel electrophoresis (horizontal electrophoresis)
- How to choose the power supply
Tips for Western Blotting
- Basic operation of Western blotting
- Method for extracting protein from tissue or cell
- Principle and workflow of Semi-dry blotting
- Western blotting (Transfer)
- How to choose the reagents for semi-dry blotting
- Transfer method according to transfer buffer and consumables
- Western blotting (Blocking)
- Western blotting (Antibody reaction and detection)
- Normalization (Western blotting data)
Imaging a Gel and a Membrane
Light Emission Detection
Liquid chromatography
References
Tips for Electrophoresis
- Basic operation of SDS-PAGE (Polyacrylamide Gel Electrophoresis)
- Method for extracting protein from tissue or cell
- Sample preparation for electrophoresis
- Preparation of polyacrylamide gel
- Polyacrylamide gel electrophoresis (PAGE)
- Principles and method of the 2-dimensional electrophoresis
- Protein electrophoresis gel staining
- Agarose gel electrophoresis (horizontal electrophoresis)
- How to choose the power supply
Tips for Western Blotting
- Basic operation of Western blotting
- Method for extracting protein from tissue or cell
- Principle and workflow of Semi-dry blotting
- Western blotting (Transfer)
- How to choose the reagents for semi-dry blotting
- Transfer method according to transfer buffer and consumables
- Western blotting (Blocking)
- Western blotting (Antibody reaction and detection)
- Normalization (Western blotting data)