Chemiluminescence Imaging System | Imaging System

WSE-6170 LuminoGraph I CMOS

Purpose and Application

Chemiluminescence imaging for Western, Southern, and Northern blotting.
Fluorescent gel imaging for gels and TLC plates stained with fluorescent dyes.
Dye-stained gel imaging for Coomassie Blue (CBB), silver-stained, and dye-colored samples.

Features

4 sensitivity setting : HQ/STD/High/Ultra
Automatic exposure : AutoExposure/Automatic merging of marker images
4 mega pixels high sensitive cooled CMOS camera enables high resolution and sensitivity
F 0.95 high sensitive lens

CS Analyzer -ATTO Original analysis software

Windows PC installation
Housekeeping protein ratio Normalization function
Free updating(Software) service
Image analysis function
Revision of distortion bands pattern
Quantitative analysis of band signal intensities

Description

① CAMERA LENS FILTER

CAMERA : COOLING CMOS CAMERA
Pixels : 2688 x 1512 (4 Mpixels)
Lens : F0.95 single-focus lens
Filter (optional) : YA-3 (LPF560), ND-0.1 (diminished filter)
The high-sensitivity cooling CMOS camera enables high-sensitivity chemiluminescence samples.
If you use the sensitivity on gain function,
“HQ” → “STD” → “HIGH” → “ULTRA”
You can increase the detection sensitivity without compromising the resolution.

② White light source (Option)

CBB-dyed gel, silver-dyed gel, etc., are used with a white transmission light source with less uniform.
It can be photographed with a high-resolution image of 4 megapixels.
Photographed images can be stored in 16-bit TIFF and are suitable for quantitative analysis.

③ Cyan Transparent Light Source (Option)

A transmission-type fluorescent irradiation device with a peak wavelength of 505 nm (half-width ± 25 nm) can detect various fluorescent staining reagents, including ethidium bromide.

Impact of Gain Settings on Detection Sensitivity and Image Quality

Unlike binning, gain-based sensitivity adjustment can increase the sensitivity itself without compromising image resolution.
It shows the results of western blotting of transferrin proteins that in a 1/2 dilution sequence from 10ng/lane with anti-transferrin antibodies. It is a photographic image of luminescence after reaction with an HRP luminescence substrate.

[Experimental conditions]
Gel : M-520L
Sample : Human Transferrin(1 ng left to 1/2 dilution sequence from lane)
Transcription Criteria: QBlot W , 24V, 15 mins
Blocking : EzBlockCAS , 30 mins
Antibodies:
Primary antibody: anti-human transferrin rabbit polyclonal antibody
Secondary antibody : HRP-labeled anti-rabbit Ig antibody
emission detection : EzWestLumi plus
Detection device : LuminoGraph I CMOS
Exposure time : 1 min

Luminescence Detection in Western Blotting with Total Protein Normalization

A sample of HeLa cell extraction protein added 0-5 ng of human albumin per lane was separated by u-PAGEL H and transcribed by QBlot Mini, and the total protein on the PVDF membrane was detected by EzStain AQua MEM.
Western blotting was performed using anti-human albumin antibodies and light emission was detected by EzWestLumi plus.
The image above shows the total protein (bright field) and target protein (luminescence) taken with LuminoGtaph I CMOS.
The graph is the result of analyzing each image data with CSanalyzer 4 and normalizing the amount of albumin based on the signal value of all proteins.
We were able to detect albumin in a wide concentration range of 3 orders, ranging from 7 pg to 5000 pg, with a signal intensity approximate to the theoretical value.
LuminoGraph I CMOS can detect low concentrations of weak luminescence with high sensitivity, and can be photographed in a wide dynamic range without saturation of high concentrations of protein.

[Experimental conditions]
Gel : UH-T420
Sample : Cell extract with human albumin added
HeLa cell extract (20μg/all lanes)
human albumin
(5 ng from left / 1/3 dilution sequence from lane)
Transcription Criteria : QBlot M, 24V, 15 mins
Blocking : EzBlockCAS, 30 mins
ANTIBODY: HRP-LABELED ANTI-HUMAN ALBUMIN
emission detection : EzWestLumi plus
Photographic equipment : LuminoGraph I CMOS
Bright field : Interior lighting, 10 ms
Luminescence : 10s

DNA Detection in Acrylamide Gel Electrophoresis

It shows the results of a 30-minute-stained gel Electrophoresis with a fluorescent reagent through a size marker of DNA.

[Experimental conditions]
Gel : EHR-T7.5L
Buffer : EzRun TG
Sample : Various DNA Molecular Weight Standards
Gel Dyeing : EzFluoroStain DNA
EzPreStain DNA ＆ RNA
Photographic equipment : LuminoGraph I CMOS
filters : YA-3 (optional)
Light Source : CyanoView (optional)
Filming time : EzFluoroStain DNA 20 ms
EzPreStain DNA ＆ RNA　50 ms

Protein Detection in Acrylamide Gel Electrophoresis

Image of a CBB dyed (EzStain AQua) gel taken using a combination of displayed light sources and filters.
The CBB stain gel was subjected to pseudo-color treatment with CS Analyzer after detection.

[Experimental conditions]
Gel : P-R16.5S (trisin gel)
Buffers: EzRun T
Sample : Chicken muscle extract, EzStandard II,
EzStandard LMW, EzProtein Ladder
Gel stain : EzStain AQua
Photographic equipment : LuminoGraph I CMOS
filters : ND-0.1 (optional)
light source : FLAT-Viewer (optional)
Exposure time : 300 ms

Brochure

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Specifications

		WSE-6170 LuminoGraph Ⅰ CMOS
Camera		High sensitivity cooled CMOS camera
Image size		2688 x 1512
Camera lens		F 0.95 prime lens
Binning		1x1, 2x2, 4x4 binning
Gradation		16-bit(65,536 gradations)
Filter		Replaceable with dedicated filter holder Filter size : 50mm square
Power		100~240 VAC, 50/60Hz, 150W (MAX)
Dimension		363 (W) × 307 (D) × 660 (H) mm
Weight		18 kg

Ordering Information

Code No.	Description	Unit
2006170	WSE-6170 LuminoGraph Ⅰ CMOS	1 set
2006276	CyanⅡ LED light kit (for WSE-6170/6270)	1 set
2006279	White LED light kit (for WSE-6170/6270)	1 set
2110030	CS Analyzer4 Windows OS	1 pk
2006177	Filter holder (for WSE-6170)	1 pk
2195931	Gel tray S (194 x 164 mm)	1 pk