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WSL-1200 KohshiFundam

WSL-1200 KohshiFundam

Purpose and Application


  • Reference Light Source calibrated with the value of light quantity by comparison with reference light source with the actual measurement of total light flux (Total light quantity)
  • Stable light emitting light source utilizing RGB LED adjusted to total light quantity of 10nW~10fW level
  • Ideal for routine checks of imaging systems and faint light measurement equipment
  • PWM (Pulse Width Modulation) controlled 11-steps
  • Support for linearity testing of optical measurement equipment
  • Free on-off and light-quantity settings for RGB colors
  • Light quantity stabilization from the heat by temperature compensation function
  • Good for reproducibility testing because the quantity of light is constant even when the ambient temperature changes


PWM (Pulse Width Modulation) controls can accurately vary up to 1/1024 of the light quantity by 1/2 each. Checked with the Luminometer with a photon-counting method and get a linear relationship between the quantity of light and the count value. The value of the RLU is graphed as 1/1,000 of the obtained 10-second count value.

By comparing the count values of the same amount of light in RGB, you can determine the color sensitivity (wavelength sensitivity) characteristics of the detector. The figure shows the relative sensitivity characteristics of the photomultiplier used in the luminometer. The photomultiplier shows that the Red light cannot have the same measurement without about 12 times the amount of light in Blue.

ATTO PHELIOS can separate and measure the intensity of each color, even though multiple colors emit light at the same time. KohshiFundam can also be used to evaluate the device. The figure is an example of verifying that the red light volume has been properly segregated and evaluated based on the filterless measurement and the PHELIOS color separation calculation, setting the red light volume to 1/1, and changing the green light volume by 1/2 in the range of 1/1 to 1/128. It has been confirmed that the color separation is good condition.


WSL-1200 KohshiFundam (for PMT)WSL-1200C KohshiFundam (for CCD)
Light sourceRGB LED
WavelengthR: 630 nm, G: 530 nm, B: 470 nm
Light quantityUp to approximately 10 pW / R·G·BUp to approximately 10 nW / R·G·B
Setting1/1 (continuous lighting) display: FULL
1/2, 1/4, 1/8, 1/16, 1/32, 1/64, 1/128, 1/256, 1/512, 1/1024 (PMW controlled)
RGB selectionfree simultaneous lighting combination
Light emissionEquivalent to aperture φ4 mm microplate E6 position
Light volume stability±2%
Environment for useTemperature: 15~45℃
Dimension86 (W) x 128 (D) x 14 (H) mm (same size as Nunc 96-well plate)
Weight190 g

Ordering Information

Code No.DescriptionUnit
3700005WSL-1200 KohshiFundam1 ea
3700006WSL-1200C KohshiFundam (for CCD camera)1 ea

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