WSE-7135 EzPreStain DNA & RNA

Purpose and Application

Fluorescent detection reagents for nucleic acids such as DNA and RNA

Features

Detection of nucleic acids (DNA/RNA) after agarose electrophoresis
Detectable immediately after electrophoresis by adding to the gel during preparation.
Detectable immediately when added to the running buffer (pre-stain).
Can also be used as a post-stain with TAE, TBE, or TE.
Use at a 1:10,000 dilution in common buffers.

Data

Gel : 1.2% agarose in 1× TAE
Running Buffer : 1× TAE
Electrophoresis : Submerge Mini
Condition : 100 V, 30 min
Sample : EzLadder DNA, 5 µL serial dilution (1/2)
Staining :
EzPreStain DNA & RNA
(1/10,000 dilution in 1× TAE, 30 min)
EtBr (5 µg/mL in 1× TAE, 30 min)
Detection : Printgraph Classic (UV, Cyan)

Instruction Manual

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Specifications

	WSE-7135 EzPreStain DNA&RNA
Major components	DMSO, fluorescent dye
Package	500 μL (10,000× concentrate)
Preparation	◆ Pre-staining 1. Add to agarose gel solution at a 1:10,000 ratio during gel preparation. 2. Dilute 1:10,000 in TAE, TBE, or TE and add to the running buffer. ◆ Post-staining 1. Prepare a 1:10,000 staining solution using TAE, TBE, or TE.
Usage	◆ Pre-staining 1. Perform electrophoresis with agarose gel containing EzPreStain DNA & RNA, then detect immediately. 2. Perform electrophoresis using running buffer containing EzPreStain DNA & RNA, then detect immediately. ◆ Post-staining 1. Immerse the gel in the prepared staining solution for 10–30 min after electrophoresis. - No destaining required. - Do not use glass containers (dye may adhere).
Detection	Recommended Light Sources Excitation (Ex): 250–320 nm (UV), 440–500 nm (Blue LED), 470–520 nm (Cyan LED) Absorption peaks: 250 nm, 482 nm Emission (Em): Max 509 nm Recommended filter: 500–550 nm long-pass filter
Storage	1 year at -20℃ in dark (unopened)