Purpose and Application
- Buffer for electrophoresis of DNA
- Sterilized, DNase free
- Suitable for analyzing low molecular weight DNA fragments
- Storable for 1 year at room temperature
Tips for Agarose Gel Electrophoresis
The type of buffers used in agarose gel electrophoresis can be determined by the size of the DNA fragment and the content of the post-migration experiment.
If you want to isolate large DNA greater than or equal to 15,000 bp, use the TAE buffer. Use a TBE buffer if you don’t want to extract DNA or don’t care about recovery rates after migration with smaller DNA of 1,000 bp or less.
The above diagram shows the results of an EzDNA Ladder applied to a 1.5% agarose gel using EzRunTAE or EzRunTBE buffers and electrophoresis at 150V for 20 minutes using a Submerge-Mini. As you can see, the TAE buffer is suitable for polymer separation and the TBE buffer is suitable for low molecular separation.
|WSE-7051 EzRun TBE|
|Major components||Tris, Boric acid, EDTA, 10x concentrated solution|
|Preparation||10x dilute with distilled water|
|Applicable amount||For 5L, 220 mL per 1 run (About 22 runs with ATTO Submerge Mini chamber)|
|Storage||For 1 year at room temperature (unopened)|
|2332392||WSE-7051 EzRun TBE||1 pk|