042-822-1117 info@attokorea.co.kr
PAGEL

Purpose and Application

  • Separation and detection of proteins
  • Second step of 2D electrophoresis
  • SDS-PAGE, Native-PAGE
  • Detection of PCR products, separation of DNA
  • Screening
  • Experimental training for students or researchers

Features

  • Mini size (90 mm x 78 mm)
  • Offers stability and reproducibility of separation
  • Glass plates with good thermal stability and uniformity employed
  • Easy to set on to the apparatus and take off the gel from the glass plate
  • Varieties of gels are available, including wide range of gel percentage
  • If you purchase more than 1 pk/month, you can rent the AE-6530 mPAGE for free!
  • Compatible electrophoresis apparatus: ATTO Mini Gel Size Electrophoresis System (WSE-1150P PageRunAce, WSE-1100P PageRun-R, AE-6531P PageRun, AE-6530P mPAGE, AE-6500 Dual Mini Slab, WSE-1165 Mini-Slab)
    ※This gel cast includes plates whose total thickness is 5 mm, so Plate holder for PAGEL are required.

Data

Pattern data of protein samples (SDS-PAGE)

Gel: SPG-520L (5~20%, 12-well gel)

Sample: M.W. marker etc.

Running buffer: AE-1410 EzRun (25 mM Tris, 192 mM Glycine, 0.1% SDS)

Electrophoresis system: AE-6530 mPAGE

Condition: Constant current 20 mA, 80 min

Staining: AE-1340 EzStain AQua (CBB staining)

Pattern data of Protein samples (Native-PAGE)

Gel: NPG-520L (5~20%, 12-well gel)

Sample: Protein standard substance samples etc.

Running buffer: WSE-7055 EzRunTG (25 mM Tris, 192 mM Glycine)

Electrophoresis system: AE-6530 mPAGE

Condition: Constant current 20 mA, 80 min

Staining: EtBr staining

Specifications

 NPU-5L PAGELNPG-310L PAGELNPG/SPG-520L PAGEL
UsagePolyacrylamide gel electrophoresis of protein or nucleic acid (SDS-PAGE, native PAGE etc)
Glass plate size120 (W) x 100 (H) mm
Gel size90 (W) x 83 (H) mm, thickness 1 mm
Gel/buffer composition5% polyacrylamide gel3~10% Gradient gel5~20% Gradient gel
Sample wells12-wells (width 4.5 mm)
Separation range50~600 kDa
250~4000 bp
20~600 kDa
50~4000 bp
10~400 kDa
20~2000 bp
sample loading volumemaximum 28 μL/well
Storage6 months when stored in a refrigerator (NOT to be frozen)
Package10 plates/box

* Gels do NOT contain SDS. Electrophoresis with buffer solution containing 24mM Tris + 192mM Glycine + 0.1% SDS (AE-1410 EzRun) is Laemmli method, and one without SDS (WSE-7055 EzRun TG) is Ornstein-Davis method.

* Use buffer solution containing 25mM Tris and 192mM Glycine or our product, [WSE-7055 EzRunTG] for DNA electrophoresis.

* PAGEL and e-PAGEL have slightly different gel making methods, so the migration patterns are not exactly the same.

Ordering Information

Code No.DescriptionUnit
2331192NPU-5L PAGEL 5% 12-well 10 gels1 pk
2331194NPG-310L PAGEL 3~10% 12-well 10 gels1 pk
2331150NPG-520L PAGEL 5~20% 12-well 10 gels1 pk
2331120SPG-520L PAGEL 5~20% 12-well 10 gels1 pk

Please Contact Us about the specifications other than the above. If it is possible, you can order from 4 boxes.

 

Related products

AE-1430 EzApply
AE-1430 EzApply
AE-1410 EzRun
AE-1410 EzRun
AE-1412 EzRun C+
AE-1412 EzRun C+
WSE-7055 EzRun TG
WSE-7055 EzRun TG
WSE-7065 EzRun MOPS
WSE-7065 EzRun MOPS
WSE-1165 Mini-Slab
WSE-1165 Mini-Slab
Mini-gel electrophoresis system AE-6530 mPAGE
AE-6530 mPAGE
WSE-1150 PageRunAce
WSE-1150 PageRunAce
e-PAGEL
e-PAGEL
e-PAGEL HR
e-PAGEL HR
p-PAGEL
p-PAGEL
AE-1340 EzStain AQua
AE-1340 EzStain AQua