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p-PAGEL

p-PAGEL

Purpose and Application

Best for Low molecular weight proteins and polypeptides separation

  • Separation and detection of proteins and polypeptides
  • SDS-PAGE
  • Screening
  • Experimental training for students or researchers

Features

  • Wide Separation  Range of 1,000-75,000Da
  • sharp band
  • short-term migration of about 60 minutes
  • a long year of life
  • Stable in quality and reproducibility
  • Adopting glass plates with excellent thermostatic efficiency
  • Easy to set up and remove gel
  • If you purchase more than 1 pk/month, you can rent the AE-6530 mPAGE for free!
  • Compatible electrophoresis apparatus: ATTO Mini Gel Size Electrophoresis System (WSE-1150P PageRunAce, WSE-1100P PageRun-R, AE-6531P PageRun, AE-6530P mPAGE, AE-6500 Dual Mini Slab, WSE-1165 Mini-Slab)
    ※This gel cast includes plates whose total thickness is 5 mm, so Plate holder for PAGEL are required.

Data

Pattern data of protein samples

Gel: P-T16.5S (16.5%, 14-well gel)

Sample: WSE-7025 EzStandard LMW (M.W. marker) etc.

Running buffer: AE-1415 EzRunT (Tris, Tricine, SDS)

Electrophoresis system: AE-6530 mPAGE

Condition: Constant current 20 mA, 60 min

Staining: AE-1340 EzStain AQua (CBB staining)

Tips for Low Molecular Weight Protein Electrophoresis

Electrophoresis of proteins and polypeptides with a molecular weight of around 20,000 Da or less is difficult to separate in the usual SDS-PAGE (Laemmli method), so the electrophoresis is carried out with tris-tricin buffer (AE-1415 EzRunT).

Gels and electrophoresis buffers are used exclusively for low molecular weight protein separation. The minimum time for the migration is about 65 minutes, and the migration is completed faster than before.

Low molecular weight proteins and polypeptides can spread if it is not fixed immediately after migration or not stained for a short period of time. CBB staining with AE-1340 EzStain AQua can be stained and destained for a short time using the microwave oven method.

Instruction Manual

Specifications

 P-T16.5S p-PAGEL (14-wells)P-R16.5S p-PAGEL (18-wells)
UsagePolyacrylamide gel electrophoresis of protein or polypeptide (SDS-PAGE etc)
Glass plate size120 (W) x 100 (H) mm
Gel size90 (W) x 83 (H) mm, thickness 1 mm
Gel/buffer compositionPolyacrylamide 16.5%, Tris-Tricine buffer
Separation range1,000~75,000 Da
Sample wells14-wells (width 4.2 mm)18-wells (width 2.9 mm)
sample loading volumemaximum 24 μL/wellmaximum 15 μL/well
Storage1 year when stored in a refrigerator (NOT to be frozen)
Package10 plates/box

Ordering Information

Code No.DescriptionUnit
2332265P-R16.5S p-PAGEL 18-well 10 gels1 pk
2332260P-T16.5S p-PAGEL 14-well 10 gels1 pk

Related products

AE-1430 EzApply
AE-1430 EzApply
AE-1415 EzRunT
AE-1415 EzRunT
WSE-7025 EzStandard LMW
WSE-7025 EzStandard LMW
AE-1340 EzStain AQua
AE-1340 EzStain AQua
WSE-1165 Mini-Slab
WSE-1165 Mini-Slab
Mini-gel electrophoresis system AE-6530 mPAGE
AE-6530 mPAGE
WSE-1150 PageRunAce
WSE-1150 PageRunAce