u-PAGEL H
Purpose and Application
Best for High molecular weight proteins separation
- Separation and detection of proteins up to 1,500 kDa (UH-T/R310)
- SDS-PAGE
- Native-PAGE
- Screening
- Electrophoresis for Western blotting (WB)
- DNA (e.g. PCR products) electrophoresis
Features
- Suitable for separation of polymer proteins above 500 kDa (UH-T/R5, UH-T/R310)
- 5~600kDa wide fractional range (UH-T/R420)
- Physical strength increases, even low concentration gel is hard to tear
- Long-term shelf life 1 year (from manufacturing date)
- High speed, short time (30min~) migration possible
- Polymer blotting is also good
- Wide application (SDS-PAGE, Native-PAGE, DNA)
- Tris-Glycine (-SDS) can be used as the migration buffer
Description
Easy-to-Handle
Polyacrylamide gel is a polymer in which acrylamide and N, N’-Methylenebisacrylamide (Bis), a crosslinking agent, are bonded by radical polymerization. Acrylamide forms a linear structure and the crosslinking agent acts as a bridge to form a polymer (gel) with a net structure. The lower the gel concentration, the larger the pore size, so high molecular protein separation is possible. However, Bis has low solubility, so it becomes a non-uniform gel due to clumps. Non-uniform gels fall in strength and tear easily. Therefore, the problem was that it was difficult to handle.
This time, ATTO succeeded in developing a uniform gel at low concentrations using a new crosslinking agent with higher solubility and longer bonding arms than Bis. It has excellent strength with tensile fracture stress about 1.7 times higher than Bis. In addition, with large pore size, the sample smoothly enters the gel’s net structure without clogging, showing higher separability.
Separation Range
| Type | Gel concentration | Separation range | ||
| 14-wells | 18-wells | Protein (kDa) | DNA (bp) | |
| UH-T5 | UH-R5 | 5% | 75 ~ 1,000 | 500 ~ 5,000 |
| UH-T310 | UH-R310 | 3~10% | 35 ~ 1,500 | 200 ~ 5,000 |
| UH-T420 | UH-R420 | 4~20% | 5 ~ 600 | 30 ~ 2,500 |
Data
Pattern data of protein samples
Gel: UH-T310 (3~10%, 14-well gel)
Sample: WSE-7035 EzStandard HMW, WSE-7020 EzProtein Ladder (MW marker) etc.
Running buffer: AE-1410 EzRun (Tris, Glycine, SDS)
Electrophoresis system:WSE-1165 Mini-Slab
Condition: Constant voltage 300 V, 40 min
Staining: AE-1340 EzStain AQua (CBB staining)
Pattern data of protein samples
Gel: UH-T5 (5%, 14-well gel)
Sample: WSE-7035 EzStandard HMW, WSE-7020 EzProtein Ladder (MW marker) etc.
Running buffer: AE-1410 EzRun (Tris, Glycine, SDS)
Electrophoresis system:WSE-1165 Mini-Slab
Condition: Constant voltage 300 V, 40 min
Staining: AE-1340 EzStain AQua (CBB staining)
Pattern data of protein samples
Gel: UH-T420 (4~20%, 14-well gel)
Sample: WSE-7035 EzStandard HMW, WSE-7020 EzProtein Ladder, WSE-7025 EzStandard LMW (MW marker) etc.
Running buffer: AE-1410 EzRun (Tris, Glycine, SDS)
Electrophoresis system:WSE-1165 Mini-Slab
Condition: Constant voltage 300 V, 35 min
Staining: AE-1340 EzStain AQua (CBB staining)
Pattern data of protein samples
Gel: UH-T310 (3~10%, 14-well gel)
Sample: Native PAGE marker etc.
Running buffer: WSE-7055 EzRun TG (Tris, Glycine)
Electrophoresis system:WSE-1165 Mini-Slab
Staining: AE-1340 EzStain AQua (CBB staining)
Pattern data of DNA samples
Gel: UH-T310 (3~10%, 14-well gel)
Sample: DNA ladder etc.
Running buffer: WSE-7055 EzRun TG (Tris, Glycine)
Electrophoresis system:WSE-1165 Mini-Slab
Condition: Constant current 20 mA, 90 min
Staining: WSE-7130 EzFluoroStain DNA
Detection: WSE-6300 LuminoGraph III (Blue LED excitation)
Specifications
| UH-TX u-PAGEL H (14-wells) | UH-RX u-PAGEL H (18-wells) | |
|---|---|---|
| Usage | Polyacrylamide gel electrophoresis of high molecular weight proteins (SDS-PAGE etc) | |
| Glass plate size | 120 (W) x 100 (H) mm | |
| Gel size | 90 (W) x 83 (H) mm, thickness 1 mm | |
| Gel/buffer composition | Polyacrylamide | |
| Sample wells | 14-wells (width 4.2 mm) | 18-wells (width 2.9 mm) |
 |  |
|
| sample loading volume | maximum 24 μL/well | maximum 18 μL/well |
| Storage | 1 year when stored in a refrigerator (5~10℃, NOT to be frozen) | |
| Package | 10 plates/box | |
* Gels do NOT contain SDS. Electrophoresis with buffer solution containing 24mM Tris + 192mM Glycine + 0.1% SDS (AE-1410 EzRun) is Laemmli method, and one without SDS (WSE-7055 EzRun TG) is Ornstein-Davis method.
* Use buffer solution containing 25mM Tris and 192mM Glycine or our product, [WSE-7055 EzRunTG] for DNA electrophoresis.
Ordering Information
| Code No. | Description | Unit |
|---|---|---|
| 2331300 | UH-T5 u-PAGEL H 5% 14-well 10 gels | 1 pk |
| 2331310 | UH-R5 u-PAGEL H 5% 18-well 10 gels | 1 pk |
| 2331302 | UH-T310 u-PAGEL H 3~10% 14-well 10 gels | 1 pk |
| 2331312 | UH-R310 u-PAGEL H 3~10% 18-well 10 gels | 1 pk |
| 2331304 | UH-T420 u-PAGEL H 4~20% 14-well 10 gels | 1 pk |
| 2331314 | UH-R420 u-PAGEL H 4~20% 18-well 10 gels | 1 pk |
Related products
AE-1410 EzRun
AE-1340 EzStain AQua
EzStackHMW Transfer kit
WSE-1165 Mini-Slab
AE-6530 mPAGE
WSE-1150 PageRunAce
