e-PAGEL
Purpose and Application
- Separation and detection of proteins (E-*** type)
- Second step of 2D electrophoresis (E-D** type)
- SDS-PAGE, Native-PAGE
- Detection of PCR products, separation of DNA
- Screening
- Experimental training for students or researchers
Features
- Mini size (90 mm x 83 mm)
- Offer stability and reproducibility of separation
- Glass plates with good thermal stability and uniformity employed
- Easy to set on to the apparatus and take off the gel from the glass plate
- Varieties of gels are available, including wide range of gel percentage, wells, gels for 2D electrophoresis also available
- If you purchase more than 1 pk/month, you can rent the AE-6530 mPAGE for free!
- Compatible electrophoresis apparatus: ATTO Mini Gel Size Electrophoresis System (WSE-1150P PageRunAce, WSE-1100P PageRun-R, AE-6531P PageRun, AE-6530P mPAGE, AE-6500 Dual Mini Slab, WSE-1165 Mini-Slab)
※This gel cast includes plates whose total thickness is 5 mm, so Plate holder for PAGEL are required.
Separation Range
| Type | Gel concentration | Separation range | |||
| 14-wells | 18-wells | no well (for 2-D) | Protein (Da) | DNA (bp) | |
| E-T7.5L | E-R7.5L | E-D7.5L | 7.5% | 40,000 ~ 400,000 | 250 ~ 3,000 |
| E-T10L | E-R10L | E-D10L | 10% | 25,000 ~ 300,000 | 150 ~ 2,000 |
| E-T12.5L | E-R12.5L | E-D12.5L | 12.5% | 10,000 ~ 250,000 | 70 ~ 1,800 |
| E-T15L | E-R15L | - | 15% | 5,000 ~ 200,000 | 50 ~ 1,500 |
| E-T520L | E-R520L | E-D520L | 5 ~ 20% | 5,000 ~ 400,000 | 50 ~ 3,000 |
| E-T1020L | E-R1020L | - | 10 ~ 20% | 5,000 ~ 300,000 | 50 ~ 1,800 |
Data
Pattern data of protein samples
Gel: E-T520L (5~20%, 14-well gel)
Sample: AE-1440 EzStandard (M.W. marker) etc.
Running buffer: AE-1410 EzRun (25 mM Tris, 192 mM Glycine, 0.1% SDS)
Electrophoresis system: AE-6531 PageRun
Condition: Constant current 20 mA, 80 min
Staining: AE-1340 EzStain AQua (CBB staining)
Pattern data of DNA samples
Gel: E-T520L (5~20%, 14-well gel)
Sample: DNA ladder etc.
Running buffer: WSE-7055 EzRunTG (25 mM Tris, 192 mM Glycine)
Electrophoresis system: AE-6531 PageRun
Condition: Constant current 20 mA, 80 min
Staining: EtBr staining
Instruction Manual
Specifications
| E-TXL e-PAGEL(R) 14-wells | E-RXL e-PAGEL(R) 18-wells | E-DXL e-PAGEL(R) for 2D electrophoresis | |
|---|---|---|---|
| Usage | Polyacrylamide gel electrophoresis of protein or nucleic acid (SDS-PAGE, native PAGE etc) | ||
| Glass plate size | 120 (W) x 100 (H) mm | ||
| Gel size | 90 (W) x 83 (H) mm, thickness 1 mm | ||
| Gel/buffer composition | Polyacrylamide, Tris-HCl buffer | ||
| Sample wells | 14-wells (width 4.2 mm) | 18-wells (width 2.9 mm) | no well |
 |  |  |
|
| sample loading volume | maximum 24 μL/well | maximum 15 μL/well | gel width 75 mm (depending on the first step IEF gel length) |
| Storage | 6 months when stored in a refrigerator (NOT to be frozen) | ||
| Package | 10 plates/box | ||
* Gels do NOT contain SDS. Electrophoresis with buffer solution containing 24mM Tris + 192mM Glycine + 0.1% SDS (AE-1410 EzRun) is Laemmli method, and one without SDS (WSE-7055 EzRun TG) is Ornstein-Davis method.
* Use buffer solution containing 25mM Tris and 192mM Glycine or our product, [WSE-7055 EzRunTG] for DNA electrophoresis.
Ordering Information
| Code No. | Description | Unit |
|---|---|---|
| 2331830 | E-T520L e-PAGEL 5~20% 14-well 10 gels | 1 pk |
| 2331840 | E-T1020L e-PAGEL 10~20% 14-well 10 gels | 1 pk |
| 2331800 | E-T7.5L e-PAGEL 7.5% 14-well 10 gels | 1 pk |
| 2331810 | E-T10L e-PAGEL 10% 14-well 10 gels | 1 pk |
| 2331820 | E-T12.5L e-PAGEL 12.5% 14-well 10 gels | 1 pk |
| 2331850 | E-T15L e-PAGEL 15% 14-well 10 gels | 1 pk |
| 2331730 | E-R520L e-PAGEL 5~20% 18-well 10 gels | 1 pk |
| 2331740 | E-R1020L e-PAGEL 10~20% 18-well 10 gels | 1 pk |
| 2331700 | E-R7.5L e-PAGEL 7.5% 18-well 10 gels | 1 pk |
| 2331710 | E-R10L e-PAGEL 10% 18-well 10 gels | 1 pk |
| 2331720 | E-R12.5L e-PAGEL 12.5% 18-well 10 gels | 1 pk |
| 2331750 | E-R15L e-PAGEL 15% 18-well 10 gels | 1 pk |
| 2331930 | E-D520L e-PAGEL 5~20% for 2-DE 10 gels | 1 pk |
| 2331940 | E-D7.5L e-PAGEL 7.5% for 2-DE 10 gels | 1 pk |
| 2331910 | E-D10L e-PAGEL 10% for 2-DE 10 gels | 1 pk |
| 2331920 | E-D12.5L e-PAGEL 12.5% for 2-DE 10 gels | 1 pk |
Related products
AE-1430 EzApply
AE-1410 EzRun
AE-1412 EzRun C+
WSE-7055 EzRun TG
WSE-7065 EzRun MOPS
WSE-1165 Mini-Slab
AE-6530 mPAGE
WSE-1150 PageRunAce
e-PAGEL HR
p-PAGEL
