e-PAGEL HR
Purpose and Application
- Separation and detection of proteins
- SDS-PAGE, Native-PAGE
- Detection of PCR products, separation of DNA
- Screening
- Experimental training for students or researchers
Features
e-PAGEL HR is high grade product of e-PAGEL.
- High-speed migration is possible; shortest migration time is 20 min.
- Sharp bands (especially, separation ability in low molecule domain improves)
- Expiration date of use: 1 year
- Minimizing smiling
- Hardly deform-able gradient gel
- Stable quality, High reproducibility
- Glass plates with good thermal stability
- Easy to set on the apparatus and take off the gel from the glass plate
- Varieties of gels are available, including wide range of gel percentage and wells
- If you purchase more than 1 pk/month, you can rent the AE-6530 mPAGE for free!
- Compatible electrophoresis apparatus: ATTO Mini Gel Size Electrophoresis System (WSE-1150P PageRunAce, WSE-1100P PageRun-R, AE-6531P PageRun, AE-6530P mPAGE, AE-6500 Dual Mini Slab, WSE-1165 Mini-Slab)
※This gel cast includes plates whose total thickness is 5 mm, so Plate holder for PAGEL are required.
Separation Range
Data
Pattern data of protein samples
Gel: EHR-T520L (5~20%, 14-well gel)
Sample: AE-1440 EzStandard (M.W. marker) etc.
Running buffer: AE-1410 EzRun (25 mM Tris, 192 mM Glycine, 0.1% SDS)
Electrophoresis system: AE-6530 mPAGE
Condition: Constant voltage 300 V, 30 min
Staining: AE-1340 EzStain AQua (CBB staining)
Instruction Manual
Specifications
| EHR-TXL e-PAGEL HR (14-wells) | EHR-RXL e-PAGEL HR 18-wells | |
|---|---|---|
| Usage | Polyacrylamide gel electrophoresis of protein or nucleic acid (SDS-PAGE, native PAGE etc) | |
| Glass plate size | 120 (W) x 100 (H) mm | |
| Gel size | 90 (W) x 83 (H) mm, thickness 1 mm | |
| Gel/buffer composition | Polyacrylamide, Tris-HCl buffer | |
| Sample wells | 14-wells (width 4.2 mm) | 18-wells (width 2.9 mm) |
 |  |
|
| sample loading volume | maximum 24 μL/well | maximum 15 μL/well |
| Storage | 1 year when stored in a refrigerator (NOT to be frozen) | |
| Package | 10 plates/box | |
* Gels do NOT contain SDS. Electrophoresis with buffer solution containing 24mM Tris + 192mM Glycine + 0.1% SDS (AE-1410 EzRun) is Laemmli method, and one without SDS (WSE-7055 EzRun TG) is Ornstein-Davis method.
* We recommend buffer [WSE-7065 EzRunMOPS] for high-speed migration.
* Use buffer solution containing 25mM Tris and 192mM Glycine or our product, [WSE-7055 EzRunTG] for DNA electrophoresis.
* The way of making [e-PAGEL HR] is different from that of [e-PAGEL] so that the migration pattern may not be same.
Ordering Information
| Code No. | Description | Unit |
|---|---|---|
| 2331970 | EHR-T520L e-PAGEL HR 5~20% 14-well 10 gels | 1 pk |
| 2331975 | EHR-T1020L e-PAGEL HR 10~20% 14-well 10 gels | 1 pk |
| 2331950 | EHR-T7.5L e-PAGEL HR 7.5% 14-well 10 gels | 1 pk |
| 2331955 | EHR-T10L e-PAGEL HR 10% 14-well 10 gels | 1 pk |
| 2331960 | EHR-T12.5L e-PAGEL HR 12.5% 14-well 10 gels | 1 pk |
| 2331965 | EHR-T15L e-PAGEL HR 15% 14-well 10 gels | 1 pk |
| 2332070 | EHR-R520L e-PAGEL HR 5~20% 18-well 10 gels | 1 pk |
| 2332075 | EHR-R1020L e-PAGEL HR 10~20% 18-well 10 gels | 1 pk |
| 2332050 | EHR-R7.5L e-PAGEL HR 7.5% 18-well 10 gels | 1 pk |
| 2332055 | EHR-R10L e-PAGEL HR 10% 18-well 10 gels | 1 pk |
| 2332060 | EHR-R12.5L e-PAGEL HR 12.5% 18-well 10 gels | 1 pk |
| 2332065 | EHR-R15L e-PAGEL HR 15% 18-well 10 gels | 1 pk |
Related products
AE-1430 EzApply
AE-1410 EzRun
AE-1412 EzRun C+
WSE-7055 EzRun TG
WSE-7065 EzRun MOPS
WSE-1165 Mini-Slab
AE-6530 mPAGE
WSE-1150 PageRunAce
e-PAGEL
p-PAGEL
