m-PAGEL

Purpose and Application

Separation and detection of proteins
SDS-PAGE, Native-PAGE
Detection of PCR products, separation of DNA
Screening
Experimental training for students or researchers

Features

High performance wide gel

30 wells/gel available
Fast migration: Completing migration in as little as 30 minutes
Sharp bands (especially low-molecular region separability)
1 year of use (unopened from 5 to 10℃ stored)
Smilingless gel
Stable quality, high reproducibility
Adopting glass plates with excellent thermostatic efficiency
Easy to set up and remove gel
Compatible electrophoresis apparatus: ATTO Wide Gel Size Electrophoresis System (WSE-1170 Multi Lane Gel Chamber)

Data

Pattern data of protein samples

Gel: M-520L (5~20%, 30-well gel)

Sample: AE-1440 EzStandard, WSE-7020 EzProtein Ladder (M.W. marker) etc.

Running buffer: AE-1410 EzRun (25 mM Tris, 192 mM Glycine, 0.1% SDS)

Electrophoresis system: WSE-1170 Multi Lane Gel Chamber

Condition: Constant voltage 300 V, 35 min

Staining: AE-1340 EzStain AQua (CBB staining)

Specifications

	M-520L m-PAGEL (30-wells)
Usage	Polyacrylamide gel electrophoresis of protein or nucleic acid (SDS-PAGE, native PAGE, PCR products etc)
Glass plate size	160 (W) x 100 (H) mm
Gel size	140 (W) x 80 (H) mm, thickness 1 mm
Gel/buffer composition	Polyacrylamide, Tris-HCl buffer
Separation range	Protein 5,000~400,000 Da, DNA 30~2,500 bp
Sample wells	30-wells (width 3 mm)
sample loading volume	maximum 20 μL/well
Storage	1 year when stored in a refrigerator (NOT to be frozen)
Package	6 plates/box

* Gels do NOT contain SDS. Electrophoresis with buffer solution containing 24mM Tris + 192mM Glycine + 0.1% SDS (AE-1410 EzRun) is Laemmli method, and one without SDS (WSE-7055 EzRun TG) is Ornstein-Davis method.

* We recommend buffer [WSE-7065 EzRunMOPS] for high-speed migration.

* Use buffer solution containing 25mM Tris and 192mM Glycine or our product, [WSE-7055 EzRunTG] for DNA electrophoresis.