WSE-7135 EzPreStain DNA & RNA

Purpose and Application

Fluorescent detection reagents for nucleic acids such as DNA and RNA

Features

Detection of nucleic acids (DNA/RNA) after agarose electrophoresis
Detectable immediately after electrophoresis by adding to the gel during preparation.
Detectable immediately when added to the running buffer (pre-stain).
Can also be used as a post-stain with TAE, TBE, or TE.
Use at a 1:10,000 dilution in common buffers.

Data

Gel : 1.2% agarose in 1× TAE
Running Buffer : 1× TAE
Electrophoresis : Submerge Mini
Condition : 100 V, 30 min
Sample : EzLadder DNA, 5 µL serial dilution (1/2)
Staining :
EzPreStain DNA & RNA
(1/10,000 dilution in 1× TAE, 30 min)
EtBr (5 µg/mL in 1× TAE, 30 min)
Detection : Printgraph Classic (UV, Cyan)

ATTO Protein / Nucleic Acid Staining Selection Guide

	AE-1340 EzStain AQua	AE-1360 EzStain Silver	AE-1310 EzStain Reverse	WSE-7160 EzStain AQua MEM	WSE-7130 EzFluoroStain DNA	WSE-7135 EzPreStain DNA&RNA
Application	Protein staining	Protein / Nucleic acid (DNA/RNA) staining	Protein staining	Blotting membrane staining	Nucleic acid (DNA) staining (ssDNA not stainable)	Nucleic acid (DNA/RNA) staining
Gel	Polyacrylamide gel	Agarose gel / Polyacrylamide gel	Polyacrylamide gel	-	Agarose gel / Polyacrylamide gel	Agarose gel / Polyacrylamide gel
Detection	Colorimetric (blue staining) Visualized under visible light	Colorimetric (brown staining) Imaged under bright-field (High background when TAE or EDTA-containing buffers are used)	Colorimetric (Areas other than bands appear white) Imaged under bright-field on a black sheet	Colorimetric (blue staining) Visualized under visible light	Fluorescence Ex: 270/370/497 nm, Em: 522 nm UV or Cyan LED excitation Orange filter / YA-3	Fluorescence Ex: 250/482 nm, Em: 509 nm UV or Cyan LED excitation Orange filter / YA-3
Features	Ready-to-use High-sensitivity CBB staining (10 ng protein) Low background Methanol- & acetic acid-free Staining completed within 30 min Destaining with water only	Easy-to-use High-sensitivity staining (1 ng protein, 10 pg DNA) Aldehyde-free Suitable for mass spectrometry (MS) analysis Staining completed within 55 min	Easy-to-use High-sensitivity negative staining (2 ng protein) Non-staining protein detection method (White background) Suitable for protein extraction from gels Staining completed within 25 min	Easy-to-use Rapid detection (1 min staining, 5 min destaining) Antibody reactions possible after staining Suitable for total protein normalization Higher sensitivity than Ponceau S	Easy-to-use High-sensitivity detection with low background Safe under UV / Blue(Cyan) LED excitation Lower mutagenicity than EtBr (Ames test) No destaining required	Easy-to-use Fluorescent detection of single- and double-stranded DNA/RNA Pre-staining possible (add to gel preparation or running buffer) Post-staining also possible
Volume	1 / 5 / 10 L (3 sizes available)	① S-1: Sodium thiosulfate ② S-2: Silver nitrate ③ S-3: Sodium hydroxide ④ S-4: Sodium thiosulfate, formaldehyde 50 mL each	① R-1: Boric acid, SDS ② R-2: Zinc sulfate 500 mL each	① Wash solution ② Stain solution ③ De-stain solution ④ Bleach (complete de-staining) 500 mL each	500 µL (10,000× concentrate)	500 µL (10,000× concentrate)
Preparation	Not required	Dilute each reagent 100-fold with distilled water	distilled water Mix 10 mL of each solution with 50 mL of distilled water	Except for the stain solution, mix with methanol in equal volume (Methanol not included)	Dilute 10,000-fold with TAE or TBE	Add 1/10,000 volume during gel preparation Dilute 10,000-fold with TAE or TBE
Usage	50 mL per mini-gel 20–25 mini-gels	Approx. 50 mini-gels	Approx. 50 mini-gels	20–25 mini-gels	-	-
Storage	Room temperature, 1 year	Refrigerated, protected from light, 2 years	Room temperature, protected from light, 2 years	Room temperature, 1 year	−20 °C, protected from light, 1 year	−20 °C, protected from light, 1 year

Instruction Manual

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Specifications

	WSE-7135 EzPreStain DNA&RNA
Major components	DMSO, fluorescent dye
Package	500 μL (10,000× concentrate)
Preparation	◆ Pre-staining 1. Add to agarose gel solution at a 1:10,000 ratio during gel preparation. 2. Dilute 1:10,000 in TAE, TBE, or TE and add to the running buffer. ◆ Post-staining 1. Prepare a 1:10,000 staining solution using TAE, TBE, or TE.
Usage	◆ Pre-staining 1. Perform electrophoresis with agarose gel containing EzPreStain DNA & RNA, then detect immediately. 2. Perform electrophoresis using running buffer containing EzPreStain DNA & RNA, then detect immediately. ◆ Post-staining 1. Immerse the gel in the prepared staining solution for 10–30 min after electrophoresis. - No destaining required. - Do not use glass containers (dye may adhere).
Detection	Recommended Light Sources Excitation (Ex): 250–320 nm (UV), 440–500 nm (Blue LED), 470–520 nm (Cyan LED) Absorption peaks: 250 nm, 482 nm Emission (Em): Max 509 nm Recommended filter: 500–550 nm long-pass filter
Storage	1 year at -20℃ in dark (unopened)