WSE-7423 EzBactYeastCrusher
Purpose and Application
- Protein extracting from Yeast & Bacteria for various experiments
- Immunoprecipitation
- Enzyme activity experiments
- Electrophoresis
- Western blotting etc.
Features
- Simple operation
(Mix reagent with bacterial cell, and then leave it→Centrifuge→Collect the supernatant) - Complete to prepare within 30 minutes
- Glass beads, Homogenizer, Ultrasonic crushing apparatus are unnecessary
- Absence of enzyme inhibition
- Attached with DNase, Protease inhibitor
- NOT include Amine
- Absence of inhibition of column purification (His, GST etc.)
ATTO Cell Fractionation and Protein Extraction Kit Selection Guide
| WSE-7420 EzRIPA Lysis kit | WSE-7421 EzSubcell Extract | WSE-7422 EzSubcell Fraction | WSE-7423 EzBactYeast Crusher | WSE-7424 EzProteoLysis Native |
|
|---|---|---|---|---|---|
| Application | Protein extraction from mammalian cells | Fractionation and extraction of cytosolic proteins from mammalian cells | Fractionation of nuclei and mitochondria from mammalian cells | Protein extraction from bacteria and yeast | Protein extraction from mammalian tissues, cells, and plants |
| Protein extraction | ○ | ○ | ○ (Extraction using supplied RIPA buffer, etc.) | ○ | ○ |
| Organelle fractionation | X | ○ | ○ | X | X |
| Effect on proteins | Contains ionic detergents | Contains ionic detergents | RIPA lysis buffer contains ionic detergents | No denaturation | No denaturation |
| IEF (Isoelectric Focusing) | X | X | X | ○ | ○ |
| SDS-PAGE | △ (Native PAGE not applicable) | △ (Native PAGE not applicable) | △ (Native PAGE not applicable) | ○ | ○ |
| Enzyme activity | △ (Depends on enzyme) | △ (Depends on enzyme) | △ (Depends on enzyme) | ○ | ○ |
| Immunoprecipitation | ○ | ○ | ○ | ○ | ○ |
| ELISA | ○ | ○ | ○ | ○ | ○ |
Brochure
Instruction Manual
Specifications
| WSE-7423 EzBactYeastCrusher | |
|---|---|
| Package | Yeast PreLysis buffer: 100 mL BactYeastLysis buffer: 100 mL Protease inhibitor (100X): 1 mL DNase (100X): 1 mL |
| Applicable amount | 100 samples, 50~100 mg/sample |
| Storage | Yeast PreLysis buffer, BactYeastLysis buffer: 1 year at room temperature Protease inhibitor, DNase: 1 year at -20℃ |
Ordering Information
| Code No. | Description | Unit |
|---|---|---|
| 2332339 | WSE-7423 EzBactYeastCrusher | 1 pk |
Related products
WSE-7420 EzRIPA Lysis kit
WSE-7421 EzSubcell Extract
