WSE-7420 EzRIPA Lysis kit
Purpose and Application
- Whole cell extract preparation for the following applications
Electrophoresis/ Western blotting/ Immunoprecipitation/ ELISA/ Enzyme activity/ etc.
Features
- Sample preparation for electrophoresis, immunoprecipitation, ELISA, enzyme activity experiments, etc.
- Standard set includes Protease inhibitor and Phosphatase inhibitor.
- Since EzRIPA Lysis kit is amine-free buffer that based on HEPES buffer, labeling experiments of prepared sample can be applied directly.
ATTO Cell Fractionation and Protein Extraction Kit Selection Guide
| WSE-7420 EzRIPA Lysis kit | WSE-7421 EzSubcell Extract | WSE-7422 EzSubcell Fraction | WSE-7423 EzBactYeast Crusher | WSE-7424 EzProteoLysis Native |
|
|---|---|---|---|---|---|
| Application | Protein extraction from mammalian cells | Fractionation and extraction of cytosolic proteins from mammalian cells | Fractionation of nuclei and mitochondria from mammalian cells | Protein extraction from bacteria and yeast | Protein extraction from mammalian tissues, cells, and plants |
| Protein extraction | ○ | ○ | ○ (Extraction using supplied RIPA buffer, etc.) | ○ | ○ |
| Organelle fractionation | X | ○ | ○ | X | X |
| Effect on proteins | Contains ionic detergents | Contains ionic detergents | RIPA lysis buffer contains ionic detergents | No denaturation | No denaturation |
| IEF (Isoelectric Focusing) | X | X | X | ○ | ○ |
| SDS-PAGE | △ (Native PAGE not applicable) | △ (Native PAGE not applicable) | △ (Native PAGE not applicable) | ○ | ○ |
| Enzyme activity | △ (Depends on enzyme) | △ (Depends on enzyme) | △ (Depends on enzyme) | ○ | ○ |
| Immunoprecipitation | ○ | ○ | ○ | ○ | ○ |
| ELISA | ○ | ○ | ○ | ○ | ○ |
Brochure
Instruction Manual
Specifications
| WSE-7420 EzRIPA Lysis kit | |
|---|---|
| Applicable amount | 100 samples, up to 2 x 10⁷ cell/sample |
| Storage | 1 year |
| Package | - EzRIPA Lysis buffer: 100 mL (20 mM HEPES pH 7.5, 1% IGEPAL® CA-630*, 0.1% SDS, 0.5% Deoxycholic acid, 150 mM Sodium Chloride) - Protease Inhibitor (100x): 1 mL (Aprotinin, Pepstatin A, Leupeptin) - Phosphatase Inhibitor (100X): 1 mL (Sodium fluoride, Sodium orthovanadate (V), β-sodium glycerophosphate) * IGEPAL® CA-630 is the same as NonidetP-40, and a trade mark registration of RhonePoulenc AG Co. |
Ordering Information
| Code No. | Description | Unit |
|---|---|---|
| 2332336 | WSE-7420 EzRIPA Lysis kit | 1 pk |
Related products
WSE-7421 EzSubcell Extract
WSE-7422 EzSubcell Fraction
WSE-7423 EzBactYeastCrusher
