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agarGEL

Purpose and Application

  • 2D gel electrophoresis of protein samples
  • an agarose gel for isoelectric focusing (the first dimension of 2D)

Features

  • Ready to use
  • A plenty volume of protein samples can be applied and separated
  • Protein samples of high molecular weight (100 ~ 250 kDa) can also be separated.
  • The gel can be easily removed from the column after electrophoresis
  • A high-voltage power source is unnecessary. OK with constant voltage 300V, 150-210 min.
  • Approximate separation pattern can be confirmed during fixation of the gel after electrophoresis
  • agarGEL can be stored for up to 1 month at -20℃ after 1st electrophoresis and then 2nd electrophoresis
  • Compatible electrophoresis apparatus: ATTO 2D Electrophoresis System (AE-6540B Disc Gel EP Chamber, WSE-1510 DiscRun Ace)

Description

※ The second dimension gel electrophoresis of agarGEL is suitable for the gel (PAGEL) which has a flat surface gel top without sample wells.

The second dimension gel (the front view)
Top of the gels of c-PAGEL (CD7.5/10/12.5/15/520L) and e-PAGEL (E-D7.5/10/12.5/520L) are flat without wells

The second dimension gel (the side view)
Put agarGEL (yellow) on the top of the second dimension gel (gray), and seal it tightly with agarose (light gray).
The glass plate of the second dimension gel is shown in light blue.

Operation Video

Data

1-DE: agarGEL pH 5 to 8

2-DE: e-PAGEL 12.5% (2D electrophoresis)

Specifications

 A-CX agarGELA-MX agarGEL
Column sizeφ7 mm x 75 mmφ7 mm x 100 mm
Gel sizeφ2.5 mm x 50 mmφ2.5 mm x 75 mm
ComponentAgarose, ampholyte, urea, etc.
pH rangeA-C310: pH 3 to 10
A-C38: pH 3 to 8
A-C510: pH 5 to 10
A-C58: pH 5 to 8
A-M310: pH 3 to 10
A-M38: pH 3 to 8
A-M510: pH 5 to 10
A-M58: pH 5 to 8
Sample volumemaximum 150 μLmaximum 300 μL
Storage5 months at 5 to 10℃
Package10 column gels/pk

Ordering Information

Code No.DescriptionUnit
2332100A-C310 agarGEL (pH 3-10, Φ2.5 mm x 50 mm) 10 gels1 pk
2332110A-C38 agarGEL (pH 3-8, Φ2.5 mm x 50 mm) 10 gels1 pk
2332120A-C58 agarGEL (pH 5-8, Φ2.5 mm x 50 mm) 10 gels1 pk
2332130A-C510 agarGEL (pH 5-10, Φ2.5 mm x 50 mm) 10 gels1 pk
2332200A-M310 agarGEL (pH 3-10, Φ2.5 mm x 75 mm) 10 gels1 pk
2332210A-M38 agarGEL (pH 3-8, Φ2.5 mm x 75 mm) 10 gels1 pk
2332220A-M58 agarGEL (pH 5-8, Φ2.5 mm x 75 mm) 10 gels1 pk
2332230A-M510 agarGEL (pH 5-10, Φ2.5 mm x 75 mm) 10 gels1 pk

Related products

WSE-1510 DiscRun Ace
WSE-1510 DiscRun Ace
AE-6540B Disc Gel EP Chamber
AE-6540B Disc Gel EP Chamber
AE-1435 EzApply 2D Kit
AE-1435 EzApply 2D Kit
e-PAGEL
e-PAGEL