agarGEL
Purpose and Application
- 2D gel electrophoresis of protein samples
- an agarose gel for isoelectric focusing (the first dimension of 2D)
Features
- Ready to use
- A plenty volume of protein samples can be applied and separated
- Protein samples of high molecular weight (100 ~ 250 kDa) can also be separated.
- The gel can be easily removed from the column after electrophoresis
- A high-voltage power source is unnecessary. OK with constant voltage 300V, 150-210 min.
- Approximate separation pattern can be confirmed during fixation of the gel after electrophoresis
- agarGEL can be stored for up to 1 month at -20℃ after 1st electrophoresis and then 2nd electrophoresis
- Compatible electrophoresis apparatus: ATTO 2D Electrophoresis System (AE-6540B Disc Gel EP Chamber, WSE-1510 DiscRun Ace)
Description
※ The second dimension gel electrophoresis of agarGEL is suitable for the gel (PAGEL) which has a flat surface gel top without sample wells.
The second dimension gel (the side view)
Put agarGEL (yellow) on the top of the second dimension gel (gray), and seal it tightly with agarose (light gray).
The glass plate of the second dimension gel is shown in light blue.
Operation Video
Data
1-DE: agarGEL pH 5 to 8
2-DE: e-PAGEL 12.5% (2D electrophoresis)
Specifications
| A-CX agarGEL | A-MX agarGEL | |
|---|---|---|
| Column size | φ7 mm x 75 mm | φ7 mm x 100 mm |
| Gel size | φ2.5 mm x 50 mm | φ2.5 mm x 75 mm |
| Component | Agarose, ampholyte, urea, etc. | |
| pH range | A-C310: pH 3 to 10 A-C38: pH 3 to 8 A-C510: pH 5 to 10 A-C58: pH 5 to 8 | A-M310: pH 3 to 10 A-M38: pH 3 to 8 A-M510: pH 5 to 10 A-M58: pH 5 to 8 |
| Sample volume | maximum 150 μL | maximum 300 μL |
| Storage | 5 months at 5 to 10℃ | |
| Package | 10 column gels/pk | |
Ordering Information
| Code No. | Description | Unit |
|---|---|---|
| 2332100 | A-C310 agarGEL (pH 3-10, Φ2.5 mm x 50 mm) 10 gels | 1 pk |
| 2332110 | A-C38 agarGEL (pH 3-8, Φ2.5 mm x 50 mm) 10 gels | 1 pk |
| 2332120 | A-C58 agarGEL (pH 5-8, Φ2.5 mm x 50 mm) 10 gels | 1 pk |
| 2332130 | A-C510 agarGEL (pH 5-10, Φ2.5 mm x 50 mm) 10 gels | 1 pk |
| 2332200 | A-M310 agarGEL (pH 3-10, Φ2.5 mm x 75 mm) 10 gels | 1 pk |
| 2332210 | A-M38 agarGEL (pH 3-8, Φ2.5 mm x 75 mm) 10 gels | 1 pk |
| 2332220 | A-M58 agarGEL (pH 5-8, Φ2.5 mm x 75 mm) 10 gels | 1 pk |
| 2332230 | A-M510 agarGEL (pH 5-10, Φ2.5 mm x 75 mm) 10 gels | 1 pk |
Related products
WSE-1510 DiscRun Ace
AE-6540B Disc Gel EP Chamber
AE-1435 EzApply 2D Kit
