AE-1435 EzApply 2D Kit
Purpose and Application
- Extraction of Proteins from Mammalian cell or tissue cultures
- 2D electrophoresis sample buffer
Features
- Separate extraction allows higher protein concentration per gel, spot detection is easy.
- Reducing reagent, alkylating reagent, washing buffer and stratified solutions are also incorporated into a kit
- Low running cost, while allowing short-time extraction and easy sample preparation
- Combination use with AE-1430 EzApply (sample preparation kit for SDS-PAGE) makes it applicable to SDS-PAGE as well as 2-D electrophoresis
Instruction Manual
Specifications
AE-1435 EzApply 2D Kit | |
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Major components | Wash buffer: Tris, NaCl Solution 1: Tris, Ampholine, DTT Solution 2: Urea, Thiourea, Surfactant Alkylated reagent: Ampholine, DTT Stratified solutions: Acrylamide, BPB, Urea |
Package | Solution/30 mL bottle x 2 (Wash buffer) Solution/20 mL bottle (Solution 1) Solution/10 mL bottle (Solution 2) Solution/1 mL tube (Alkylated reagent) Solution/500 μL tube (Stratified solution) Powder/tube (DTT) |
Preparation | Appropriated amount of solution is applied to tissues or cells, which were lysed and extracted |
Applicable amount | Extracted from 100 mg of mammalian tissue 20 runs |
Storage | 6 months at -20℃ (before dissolved in DTT) 1 week after dissolved in DTT |
Ordering Information
Code No. | Description | Unit |
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2332335 | AE-1435 EzApply 2D Kit | 1 pk |