WSE-7140 EzWestBlue W
Purpose and Application
- EzWestBlue is a TMB substrate for horseradish peroxidase (HRP) used for the colorimetric detection of immunoblotting
- Note: not applicable to ELISA
Features
- Substrate for horseradish peroxidase (HRP)
- Ready to use
- Highly sensitive detection comparable to chemiluminescence detection
- Visualized in bright and clear blue
- Safer than diaminobenzidine (DAB)
- Support total protein normalization
- Absence of enzyme inhibition due to the absence of organic solvent
Results
Higher sensitivity than previous AE-1490 EzWestBlue
ATTO HRP Detection Reagent Selection Guide
| WSE-7110 EzWestLumiOne | WSE-7120S EzWestLumi plus | WSE-7120L EzWestLumi plus | WSE-7140 EzWestBlue W | WSE-7145 EzELISA TMB |
|
|---|---|---|---|---|---|
| Western Blot | 〇 | 〇 | 〇 | × | |
| ELISA | △ | △ | × | 〇 | |
| Volume | 250 mL | 2 x 50 mL | 2 x 250 mL | 200 mL | 200 mL 200 mL (Stop Solution) |
| Sensitivity | Several pg/ban | Several hundred fg/band | 300 pg/band | Several pg/well | |
| Signal duration (chemiluminescence) | 1h | 3h< | - | - | |
| Preparation | Single-solution type, no preparation required | Two-solution type | Single-solution type, no preparation required | Single-solution type, no preparation required | |
| Storage temperature | 2-8°C | ||||
| Shelf life | 1 year | ||||
| Usage/ Approximate coverage | 0.05 mL/cm² 5,000 cm² | 0.05 mL/cm² 2,000 cm² | 0.05 mL/cm² 10,000 cm² | 0.1 mL/cm² 2,000 cm² | 0.1 mL/well 2,000 wells |
Instruction Manual
Specifications
| WSE-7140 EzWestBlue W | |
|---|---|
| Major components | TMB (TMB 3,3',5,5' tetramethylbenzidine) solution (not containing organic solvents) |
| Package | 200 mL/bottle |
| Preparation | Ready to use |
| Applicable amount | 0.1 mL/cm² Serve 2,000 cm² (approximately 25 sheets of mini-size gel) |
| Storage | 1 year when stored in a refrigerator |
Ordering Information
| Code No. | Description | Unit |
|---|---|---|
| 2332456 | WSE-7140 EzWestBlue W | 1 pk |
Related products
ClearBlot Membrane-P
ClearBlot P plus Membrane
WSE-7110 EzWestLumiOne
