WSE-7051 EzRun TBE

Purpose and Application

Buffer for electrophoresis of DNA

Features

Sterilized, DNase free
Suitable for analyzing low molecular weight DNA fragments
Storable for 1 year at room temperature

Tips for Agarose Gel Electrophoresis

The type of buffers used in agarose gel electrophoresis can be determined by the size of the DNA fragment and the content of the post-migration experiment.

If you want to isolate large DNA greater than or equal to 15,000 bp, use the TAE buffer. Use a TBE buffer if you don’t want to extract DNA or don’t care about recovery rates after migration with smaller DNA of 1,000 bp or less.

The above diagram shows the results of an EzDNA Ladder applied to a 1.5% agarose gel using EzRunTAE or EzRunTBE buffers and electrophoresis at 150V for 20 minutes using a Submerge-Mini. As you can see, the TAE buffer is suitable for polymer separation and the TBE buffer is suitable for low molecular separation.

ATTO Electrophoresis Buffer Selection Guide

Application	Running Buffer			Gel Buffer	Applicable Gels			Features
Target	Protein		DNA	DNA
Method	SDS-PAGE	Native-PAGE	DNA-PAGE	DNA-PAGE	Hand made Gels	ATTO Precast Gels	Agarose Gel
AE-1411 EzRun	〇	-	-	-	〇 Tris-based and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	-	A general-purpose Tris/Glycine/SDS running buffer compliant with the Laemmli method. Low cost; supplied as a powder for long-term storage.
AE-1412 EzRun C+	〇	-	-	-	〇 Tris-based and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	-	Contains reducing agents to suppress band broadening caused by re-oxidation during electrophoresis, enabling sharp band separation. Supplied as a powder.
AE-1415 EzRun T	〇	-	-	-	〇 Tricine-based polyacrylamide gels	〇 p-PAGEL, cp-PAGEL Neo	-	Tris/Tricine/SDS buffer for Tricine PAGE, suitable for separation of peptides and low–molecular-weight proteins.
WSE-7050 EzRun TAE	-	-	〇	〇			〇	Gel buffer / running buffer mainly used for DNA separation on agarose gels.
WSE-7051 EzRun TBE	-	-	〇	〇	〇 TBE-based, Tris-based, and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	〇	Gel buffer / running buffer mainly used for DNA separation. Can be used with both agarose gels and polyacrylamide gels.
WSE-7055 EzRun TG	-	〇	〇	〇	〇 Tris-based and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	-	Tris/Glycine buffer without SDS. Compatible with Native-PAGE for proteins and DNA. Enables BN-PAGE of proteins when supplemented with WSE-7067 EzBlueNative Additive.
WSE-7056 EzRun ClearNative	-	〇	-	-	〇 Tris-based and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	-	High-resolution Clear Native PAGE running buffer without SDS. Uses an anionic surfactant to minimize electrophoretic interference, allowing efficient separation of native (non-denatured) proteins.
WSE-7057 EzRun BlueNative	-	〇	-	-	〇 Tris-based and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	-	Blue Native PAGE running buffer without SDS. Supplied with EzRun BlueNative Additive. Coomassie Brilliant Blue (CBB) enables separation of native protein complexes without dissociation while minimizing electrophoretic interference.
WSE-7065 EzRun MOPS	〇		-	-	〇 Bis-Tris–based, imidazole-based, Tris-based, and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	-	Tris/MOPS/SDS buffer compatible with various polyacrylamide gels. Reduces protein mobility, improving resolution in the low–molecular-weight range. High electrophoretic speed enables rapid separation.
WSE-7066 EzRun MOPS non-SDS	-	〇	〇	〇	〇 Bis-Tris–based, imidazole-based, Tris-based, and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	〇	Tris/MOPS buffer without SDS. Compatible with Native-PAGE for proteins and DNA. Enables BN-PAGE of proteins when supplemented with WSE-7067 EzBlueNative Additive.

Instruction Manual

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Specifications

	WSE-7051 EzRun TBE
Major components	Tris, Boric acid, EDTA, 10x concentrated solution
Package	500 mL/bottle
Preparation	10x dilute with distilled water
Applicable amount	For 5L, 220 mL per 1 run (About 22 runs with ATTO Submerge Mini chamber)
Storage	For 1 year at room temperature (unopened)