agarGEL
Purpose and Application
- 2D gel electrophoresis of protein samples
- an agarose gel for isoelectric focusing (the first dimension of 2D)
Features
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Compatible with mini-slab gel systems
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Ready-to-use; no swelling or pretreatment required
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Allows loading of large sample amounts detectable by CBB staining
Separates high–molecular-weight proteins (100–250 kDa)
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Easy gel removal after electrophoresis by peeling off the bottom dialysis membrane
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Precast gel ensures stable quality and high reproducibility
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Electrophoresis completed in ~3 hours at constant 300 V
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Approximate band pattern visible during fixation
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Can be stored frozen for up to one month after electrophoresis
- Compatible electrophoresis apparatus: ATTO 2D Electrophoresis System (AE-6540B Disc Gel EP Chamber, WSE-1510 DiscRun Ace)
Description
Data
Electrophoresis system: 2D mini-slab gel system
Sample: Escherichia coli (approx. 90 mg wet weight)
Protein extraction: EzApply2DKit; ultrasonic lysis using a hydrophobic lysis solution
First dimension: A-M310 agarose gel
Second dimension: E-D520Le Pagel
Detection: AE-1340 EzStain AQua
Operation Video
Specifications
| A-M310/38/58/510agarGEL | |
|---|---|
| Application | Isoelectric focusing (first dimension of 2D electrophoresis) |
| Column size | Φ 7 × 100 mm Material: glass |
| Gel size | Φ 2.5 × 75 mm |
| Component | Mainly agarose, ampholytes, and urea |
| pH range | A-M310: pH 3 to 10 A-M38: pH 3 to 8 A-M510: pH 5 to 10 A-M58: pH 5 to 8 |
| Sample volume | maximum 300 μL |
| Storage | 5 months under refrigerated, sealed storage (from date of manufacture) |
| Package | 10 gels per box |
Ordering Information
| Code No. | Description | Unit |
|---|---|---|
| 2332200 | A-M310 agarGEL (pH 3-10, Φ2.5 mm x 75 mm) 10 gels | 1 pk |
| 2332210 | A-M38 agarGEL (pH 3-8, Φ2.5 mm x 75 mm) 10 gels | 1 pk |
| 2332220 | A-M58 agarGEL (pH 5-8, Φ2.5 mm x 75 mm) 10 gels | 1 pk |
| 2332230 | A-M510 agarGEL (pH 5-10, Φ2.5 mm x 75 mm) 10 gels | 1 pk |
Related products
WSE-1510 DiscRun Ace
AE-6540B Disc Gel EP Chamber
AE-1435 EzApply 2D Kit
