WSE-7422 EzSubcell Fraction
Purpose and Application
- Electrophoresis/ Western blotting/ Immunoprecipitation/ ELISA/ Bioactivity/Protein purification/ Chromatin immunoprecipitation assay/ Enzyme activity/ etc.
Features
- Sample preparation for electrophoresis, immunoprecipitation, ELISA, chromatin immunoprecipitation assay, enzyme activity & bioactivity experiments, etc.
- Simple preparation of organella fractionation in high reproducibility and purity.
- Additionally required material is only cooled centrifuge( to 10,000 x g).
- Process for all fractionation is completed in 2 hours.
- Detergent or non-detergent method is available.
ATTO Cell Fractionation and Protein Extraction Kit Selection Guide
| WSE-7420 EzRIPA Lysis kit | WSE-7421 EzSubcell Extract | WSE-7422 EzSubcell Fraction | WSE-7423 EzBactYeast Crusher | WSE-7424 EzProteoLysis Native |
|
|---|---|---|---|---|---|
| Application | Protein extraction from mammalian cells | Fractionation and extraction of cytosolic proteins from mammalian cells | Fractionation of nuclei and mitochondria from mammalian cells | Protein extraction from bacteria and yeast | Protein extraction from mammalian tissues, cells, and plants |
| Protein extraction | ○ | ○ | ○ (Extraction using supplied RIPA buffer, etc.) | ○ | ○ |
| Organelle fractionation | X | ○ | ○ | X | X |
| Effect on proteins | Contains ionic detergents | Contains ionic detergents | RIPA lysis buffer contains ionic detergents | No denaturation | No denaturation |
| IEF (Isoelectric Focusing) | X | X | X | ○ | ○ |
| SDS-PAGE | △ (Native PAGE not applicable) | △ (Native PAGE not applicable) | △ (Native PAGE not applicable) | ○ | ○ |
| Enzyme activity | △ (Depends on enzyme) | △ (Depends on enzyme) | △ (Depends on enzyme) | ○ | ○ |
| Immunoprecipitation | ○ | ○ | ○ | ○ | ○ |
| ELISA | ○ | ○ | ○ | ○ | ○ |
Instruction Manual
Specifications
| WSE-7422 EzSubcell Fraction | |
|---|---|
| Components | Extraction buffer: HEPES, detergent Protease Inhibitor (100X): Aprotinin, Pepstatin A, Leupeptin |
| Applicable amounts | 50 samples, up to 2 x 10⁷ cell/sample |
| Storage | 1 year |
| Package | Fraction buffer 1: 50 mL Fraction buffer 2: 50 mL RIPA Lysis buffer: 20 mL Detergent mix (50X): 1 mL Protease Inhibitor (100X): 0.7 mL |
Ordering Information
| Code No. | Description | Unit |
|---|---|---|
| 2332338 | WSE-7422 EzSubcell Fraction | 1 pk |
Related products
WSE-7420 EzRIPA Lysis kit
WSE-7421 EzSubcell Extract
WSE-7423 EzBactYeastCrusher
