WSE-7160 EzStain AQua MEM

Purpose and Application

Staining and destaining solution kit for detecting proteins on the membrane after blotting
Before the antibody reaction, carry out staining to confirm the blotting efficiency
The result can be used to normalize the target protein after the antibody reaction

Features

Can detect proteins on membrane
Staining and destaining in a short time
Bright blue band
Antigens and antibodies can be reacted after destaining
Support for normalization of total protein
Sensitivity is higher than Ponceau S
No acetic acid odor

Results

1. Membrane staining after blotting

The transferred membrane was cut in half, one stained by a conventional staining method (Ponceau S) (A), and the other stained and destained with WSE-7160 EzStain AQua MEM (B).
As for the sample, loaded a 3/4 serial dilution of Hela cell extract. WSE-7160 EzStain AQua MEM was detected at all concentrations.

2. Antibody reaction after staining

After the blotting, the membrane was cut in halt, one carried out an antibody reaction under normal operation to detect chemiluminescence (A).
And the other membrane was stained and destained with WSE-7160 EzStain AQua MEM, and an antibody reaction was performed to detect chemiluminescence (B).
It was shown that the antibody reaction does not affected by staining as the both membrane have the same sensitivity and patterns.

Operation Video

ATTO Protein / Nucleic Acid Staining Selection Guide

	AE-1340 EzStain AQua	AE-1360 EzStain Silver	AE-1310 EzStain Reverse	WSE-7160 EzStain AQua MEM	WSE-7130 EzFluoroStain DNA	WSE-7135 EzPreStain DNA&RNA
Application	Protein staining	Protein / Nucleic acid (DNA/RNA) staining	Protein staining	Blotting membrane staining	Nucleic acid (DNA) staining (ssDNA not stainable)	Nucleic acid (DNA/RNA) staining
Gel	Polyacrylamide gel	Agarose gel / Polyacrylamide gel	Polyacrylamide gel	-	Agarose gel / Polyacrylamide gel	Agarose gel / Polyacrylamide gel
Detection	Colorimetric (blue staining) Visualized under visible light	Colorimetric (brown staining) Imaged under bright-field (High background when TAE or EDTA-containing buffers are used)	Colorimetric (Areas other than bands appear white) Imaged under bright-field on a black sheet	Colorimetric (blue staining) Visualized under visible light	Fluorescence Ex: 270/370/497 nm, Em: 522 nm UV or Cyan LED excitation Orange filter / YA-3	Fluorescence Ex: 250/482 nm, Em: 509 nm UV or Cyan LED excitation Orange filter / YA-3
Features	Ready-to-use High-sensitivity CBB staining (10 ng protein) Low background Methanol- & acetic acid-free Staining completed within 30 min Destaining with water only	Easy-to-use High-sensitivity staining (1 ng protein, 10 pg DNA) Aldehyde-free Suitable for mass spectrometry (MS) analysis Staining completed within 55 min	Easy-to-use High-sensitivity negative staining (2 ng protein) Non-staining protein detection method (White background) Suitable for protein extraction from gels Staining completed within 25 min	Easy-to-use Rapid detection (1 min staining, 5 min destaining) Antibody reactions possible after staining Suitable for total protein normalization Higher sensitivity than Ponceau S	Easy-to-use High-sensitivity detection with low background Safe under UV / Blue(Cyan) LED excitation Lower mutagenicity than EtBr (Ames test) No destaining required	Easy-to-use Fluorescent detection of single- and double-stranded DNA/RNA Pre-staining possible (add to gel preparation or running buffer) Post-staining also possible
Volume	1 / 5 / 10 L (3 sizes available)	① S-1: Sodium thiosulfate ② S-2: Silver nitrate ③ S-3: Sodium hydroxide ④ S-4: Sodium thiosulfate, formaldehyde 50 mL each	① R-1: Boric acid, SDS ② R-2: Zinc sulfate 500 mL each	① Wash solution ② Stain solution ③ De-stain solution ④ Bleach (complete de-staining) 500 mL each	500 µL (10,000× concentrate)	500 µL (10,000× concentrate)
Preparation	Not required	Dilute each reagent 100-fold with distilled water	distilled water Mix 10 mL of each solution with 50 mL of distilled water	Except for the stain solution, mix with methanol in equal volume (Methanol not included)	Dilute 10,000-fold with TAE or TBE	Add 1/10,000 volume during gel preparation Dilute 10,000-fold with TAE or TBE
Usage	50 mL per mini-gel 20–25 mini-gels	Approx. 50 mini-gels	Approx. 50 mini-gels	20–25 mini-gels	-	-
Storage	Room temperature, 1 year	Refrigerated, protected from light, 2 years	Room temperature, protected from light, 2 years	Room temperature, 1 year	−20 °C, protected from light, 1 year	−20 °C, protected from light, 1 year

Instruction Manual

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Specifications

	WSE-7160 EzStain AQua MEM
Major components	Staining solution: Coomassie Brilliant Blue (CBB)
Package	Wash (Pretreatment solution): 500 mL Stain (Staining solution): 500 mL De-Stain (Destaining solution): 500 mL Bleach (Compelete destaining solution): 500 mL
Preparation	Wash: 2× dilute with methanol De-Stain: 2× dilute with methanol Bleach: 2× dilute with methanol
Applicable amount	0.2~0.3 mL/cm² 20~25 sheets in mini size gel (80 × 95 mm)
Storage	For 1 year at room temperature