QBlot kit
![WSE-4057 QBlot kit M WSE-4057 QBlot kit M](https://www.attokorea.co.kr/wp-content/uploads/2020/12/WSE-4057-QBlot-kit-M.png)
Purpose and Application
- Semi-Dry blotting
- Pre-wet PVDF membrane & filter paper
Features
- Western blotting is needed a workflow for equilibrating membrane and filter paper. Normally, the equilibration is taken 15~30 min. QBlot kit is needless the equilibration and it may use for western blotting immediately.
- ATTO made shortest time for transfer 5-10 min with good transfer efficiency.
- QBlot kit not only ATTO Semi-dry blotting system but also, may use other semi-dry transfer system as well.
- Three different sizes
Compact gel size: WSE-4056 QBlot kit C (65×63 cm)
Mini gel size: WSE-4057 QBlot kit M (90×85 mm)
Wide gel size: WSE-4058 QBlot kit W (145×85 mm) - Improved filter paper and transfer buffer
- A sample is available. Please request the sample through the “Contact Us” menu at the top.
Description
![4055_sandwich](https://www.attokorea.co.kr/wp-content/uploads/2020/03/4055_sandwich.png)
*Ready-to-Use
Bottom stack: Filter paper immersed in the transfer buffer
Hydrophilized / Equilibrated PVDF membrane
Top stack: Filter paper immersed in the transfer buffer
Just cut off the QBlot kit and sets up right away.
No need to prepare transfer buffer, filter paper, and membrane.
![QBlot kit_pros](https://www.attokorea.co.kr/wp-content/uploads/2020/12/QBlot-kit_pros-scaled.jpg)
Comparison with transfer buffers
![transfer buffer_selection](https://www.attokorea.co.kr/wp-content/uploads/2020/03/transfer-buffer_selection.png)
Operation Guide
![web_-1_large](https://www.attokorea.co.kr/wp-content/uploads/2020/03/web_-1_large.jpg)
1. Take “Bottom Stack” out
![web_-2_large](https://www.attokorea.co.kr/wp-content/uploads/2020/03/web_-2_large.jpg)
2. Rolling it to removing a bubble
![web_-3_large](https://www.attokorea.co.kr/wp-content/uploads/2020/03/web_-3_large.jpg)
3. Layout it with sample gel
![web_-4_large](https://www.attokorea.co.kr/wp-content/uploads/2020/03/web_-4_large.jpg)
4. Rolling it to removing a bubble
![web_-6_large](https://www.attokorea.co.kr/wp-content/uploads/2020/03/web_-6_large.jpg)
5. Take “Top Stack” out and overlay it.
![web_-7_large](https://www.attokorea.co.kr/wp-content/uploads/2020/03/web_-7_large.jpg)
6. Rolling it to removing a bubble.
7. Set “Top Electrode cover (ATTO blotting system)” and start “Blotting”
Transfer condition
Blotting condition | |||
---|---|---|---|
WSE-4056 QBlot kit C | WSE-4057 QBlot kit M | WSE-4058 QBlot kit W | |
High speed (C.V.) | 24 V (200~400 mA) 5~10 min | 24 V (500~800 mA) 5~10min | 24 V (700~1,100 mA) 5~10 min |
High speed (C.C.) | 300 mA/gel (15~25 V) 5~10 min | 600 mA/gel (15~25 V) 5~10 min | 900 mA/gel (15~25 V) 5~10 min |
Standard (C.V.) | 12 V (100~200 mA) 15~30 min | 12 V (100~400 mA) 15~30 min | 12 V (200~600 mA) 15~30 min |
Standard (C.C.) | 150 mA/gel (5~15 V) 15~30 min | 250 mA/gel (5~15 V) 15~30 min | 300 mA/gel (5~15 V) 15~30 min |
Operation Video
Specifications
WSE-4056 QBlot kit C | WSE-4057 QBlot kit M | WSE-4058 QBlot kit W | |
---|---|---|---|
Composition | Top Stack (Filter paper) 65 x 63 mm 10 pk Bottom Stack (PVDF membrane and filter paper) 65 x 63 mm 10 pk Gel Wash buffer (5x) 100 mL | Top Stack (Filter paper) 90 x 85 mm 10 pk Bottom Stack (PVDF membrane and filter paper) 90 x 85 mm 10 pk Gel Wash buffer (5x) 100 mL | Top Stack (Filter paper) 145 x 85 mm 6 pk Bottom Stack (PVDF membrane and filter paper) 145 x 85 mm 6 pk Gel Wash buffer (5x) 100 mL |
Material | PVDF membrane: WSE-4051 ClearBlot P plus Membrane (pore size: 0.2 μm) Filter paper: non-woven (High quality absorbency) for QBlot Kit only |
||
Storage | 1 year at cold storage | ||
Applicable amount | 10 stacks/box (1 stack: 1 pouch bag of Top Stack and 1 pouch bag of Bottom Stack) | 10 stacks/box (1 stack: 1 pouch bag of Top Stack and 1 pouch bag of Bottom Stack) | 6 stacks/box (1 stack: 1 pouch bag of Top Stack and 1 pouch bag of Bottom Stack) |
Ordering Information
Code No. | Description | Unit |
---|---|---|
2322441 | WSE-4056 QBlot kit C | 1 pk |
2322443 | WSE-4057 QBlot kit M | 1 pk |
2322447 | WSE-4058 QBlot kit W | 1 pk |