QBlot kit
Purpose and Application
- Semi-Dry blotting
- Pre-wet PVDF membrane & filter paper
Features
- Western blotting is needed a workflow for equilibrating membrane and filter paper. Normally, the equilibration is taken 15~30 min. QBlot kit is needless the equilibration and it may use for western blotting immediately.
- ATTO made shortest time for transfer 5-10 min with good transfer efficiency.
- QBlot kit not only ATTO Semi-dry blotting system but also, may use other semi-dry transfer system as well.
- Three different sizes
Compact gel size: WSE-4056 QBlot kit C (65×63 cm)
Mini gel size: WSE-4057 QBlot kit M (90×85 mm)
Wide gel size: WSE-4058 QBlot kit W (145×85 mm) - Improved filter paper and transfer buffer
- A sample is available. Please request the sample through the “Contact Us” menu at the top.
Description
*Ready-to-Use
Bottom stack: Filter paper immersed in the transfer buffer
Hydrophilized / Equilibrated PVDF membrane
Top stack: Filter paper immersed in the transfer buffer
Just cut off the QBlot kit and sets up right away.
No need to prepare transfer buffer, filter paper, and membrane.
Comparison with transfer buffers
Operation Guide
1. Take “Bottom Stack” out
2. Rolling it to removing a bubble
3. Layout it with sample gel
4. Rolling it to removing a bubble
5. Take “Top Stack” out and overlay it.
6. Rolling it to removing a bubble.
7. Set “Top Electrode cover (ATTO blotting system)” and start “Blotting”
Transfer condition
| Blotting condition | |||
|---|---|---|---|
| WSE-4056 QBlot kit C | WSE-4057 QBlot kit M | WSE-4058 QBlot kit W | |
| High speed (C.V.) | 24 V (200~400 mA) 5~10 min | 24 V (500~800 mA) 5~10min | 24 V (700~1,100 mA) 5~10 min |
| High speed (C.C.) | 300 mA/gel (15~25 V) 5~10 min | 600 mA/gel (15~25 V) 5~10 min | 900 mA/gel (15~25 V) 5~10 min |
| Standard (C.V.) | 12 V (100~200 mA) 15~30 min | 12 V (100~400 mA) 15~30 min | 12 V (200~600 mA) 15~30 min |
| Standard (C.C.) | 150 mA/gel (5~15 V) 15~30 min | 250 mA/gel (5~15 V) 15~30 min | 300 mA/gel (5~15 V) 15~30 min |
Operation Video
Specifications
| WSE-4056 QBlot kit C | WSE-4057 QBlot kit M | WSE-4058 QBlot kit W | |
|---|---|---|---|
| Composition | Top Stack (Filter paper) 65 x 63 mm 10 pk Bottom Stack (PVDF membrane and filter paper) 65 x 63 mm 10 pk Gel Wash buffer (5x) 100 mL | Top Stack (Filter paper) 90 x 85 mm 10 pk Bottom Stack (PVDF membrane and filter paper) 90 x 85 mm 10 pk Gel Wash buffer (5x) 100 mL | Top Stack (Filter paper) 145 x 85 mm 6 pk Bottom Stack (PVDF membrane and filter paper) 145 x 85 mm 6 pk Gel Wash buffer (5x) 100 mL |
| Material | PVDF membrane: WSE-4051 ClearBlot P plus Membrane (pore size: 0.2 μm) Filter paper: non-woven (High quality absorbency) for QBlot Kit only |
||
| Storage | 1 year at cold storage | ||
| Applicable amount | 10 stacks/box (1 stack: 1 pouch bag of Top Stack and 1 pouch bag of Bottom Stack) | 10 stacks/box (1 stack: 1 pouch bag of Top Stack and 1 pouch bag of Bottom Stack) | 6 stacks/box (1 stack: 1 pouch bag of Top Stack and 1 pouch bag of Bottom Stack) |
Ordering Information
| Code No. | Description | Unit |
|---|---|---|
| 2322441 | WSE-4056 QBlot kit C | 1 pk |
| 2322443 | WSE-4057 QBlot kit M | 1 pk |
| 2322447 | WSE-4058 QBlot kit W | 1 pk |
Related products
WSE-4045 HorizeBLOT 4M
WSE-4025 HorizeBLOT 2M
WSE-4125 PoweredBLOT 2M
