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QBlot kit

WSE-4057 QBlot kit M

Purpose and Application

  • Semi-Dry blotting
  • Pre-wet PVDF membrane & filter paper

Features

  • Western blotting is needed a workflow for equilibrating membrane and filter paper. Normally, the equilibration is taken 15~30 min. QBlot kit is needless the equilibration and it may use for western blotting immediately.
  • ATTO made shortest time for transfer 5-10 min with good transfer efficiency.
  • QBlot kit not only ATTO Semi-dry blotting system but also, may use other semi-dry transfer system as well.
  • Three different sizes
    Compact gel size: WSE-4056 QBlot kit C (65×63 cm)
    Mini gel size: WSE-4057 QBlot kit M (90×85 mm)
    Wide gel size: WSE-4058 QBlot kit W (145×85 mm)
  • Improved filter paper and transfer buffer
  • A sample is available. Please request the sample through the “Contact Us” menu at the top.

Description

*Ready-to-Use

Bottom stack: Filter paper immersed in the transfer buffer
Hydrophilized / Equilibrated PVDF membrane

Top stack: Filter paper immersed in the transfer buffer

Just cut off the QBlot kit  and sets up right away.
No need to prepare transfer buffer, filter paper, and membrane.

Comparison with transfer buffers

Operation Guide

1. Take “Bottom Stack” out

2. Rolling it to removing a bubble

3. Layout it with sample gel

4. Rolling it to removing a bubble

5. Take “Top Stack” out and overlay it.

6. Rolling it to removing a bubble.

7. Set “Top Electrode cover (ATTO blotting system)” and start “Blotting”

Transfer condition

Blotting condition
WSE-4056 QBlot kit CWSE-4057 QBlot kit MWSE-4058 QBlot kit W
High speed (C.V.)24 V (200~400 mA)
5~10 min
24 V (500~800 mA)
5~10min
24 V (700~1,100 mA)
5~10 min
High speed (C.C.)300 mA/gel (15~25 V)
5~10 min
600 mA/gel (15~25 V)
5~10 min
900 mA/gel (15~25 V)
5~10 min
Standard (C.V.)12 V (100~200 mA)
15~30 min
12 V (100~400 mA)
15~30 min
12 V (200~600 mA)
15~30 min
Standard (C.C.)150 mA/gel (5~15 V)
15~30 min
250 mA/gel (5~15 V)
15~30 min
300 mA/gel (5~15 V)
15~30 min

Operation Video

Specifications

 WSE-4056 QBlot kit CWSE-4057 QBlot kit MWSE-4058 QBlot kit W
CompositionTop Stack (Filter paper) 65 x 63 mm 10 pk
Bottom Stack (PVDF membrane and filter paper) 65 x 63 mm 10 pk
Gel Wash buffer (5x) 100 mL
Top Stack (Filter paper) 90 x 85 mm 10 pk
Bottom Stack (PVDF membrane and filter paper) 90 x 85 mm 10 pk
Gel Wash buffer (5x) 100 mL
Top Stack (Filter paper) 145 x 85 mm 6 pk
Bottom Stack (PVDF membrane and filter paper) 145 x 85 mm 6 pk
Gel Wash buffer (5x) 100 mL
MaterialPVDF membrane: WSE-4051 ClearBlot P plus Membrane (pore size: 0.2 μm)
Filter paper: non-woven (High quality absorbency) for QBlot Kit only
Storage1 year at cold storage
Applicable amount10 stacks/box (1 stack: 1 pouch bag of Top Stack and 1 pouch bag of Bottom Stack)10 stacks/box (1 stack: 1 pouch bag of Top Stack and 1 pouch bag of Bottom Stack)6 stacks/box (1 stack: 1 pouch bag of Top Stack and 1 pouch bag of Bottom Stack)

Ordering Information

Code No.DescriptionUnit
2322441WSE-4056 QBlot kit C1 pk
2322443WSE-4057 QBlot kit M1 pk
2322447WSE-4058 QBlot kit W1 pk

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