WSE-7145 EzELISA TMB
Purpose and Application
- EzELISA TMB is an ELISA detection kit that includes a color reagent for HRP (TMB solution) and a stop solution.
Features
- For use in ELISA assays
- Includes TMB solution (color reagent for HRP)
- Kit contains both TMB and stop solution
- A sample is available. Please request the sample through the “Contact Us” menu at the top.
Description
Easy operation
- Add 100 μL of TMB solution to each well after completing the antigen-antibody reaction and washing. Incubate for 5–30 minutes to allow color development.
→ The solution will turn blue. - Add 100 μL of STOP solution to each well.
→ The solution will turn yellow. - Measure the absorbance at 450 nm.
Absorbance Spectrum of EzELISA TMB
- When TMB solution is added and the enzymatic reaction begins, the solution turns blue, showing maximum absorbance at 370 nm and 652 nm.
- Upon addition of the stop solution, the reaction is terminated, and the color immediately shifts to yellow, with a peak absorbance at 450 nm.
Brochure
Specifications
| WSE-7145 EzELISA TMB | |
|---|---|
| Composition | 1. TMB Solution (HRP Chromogenic Reagent): 200 mL 2. STOP Solution: 200 mL |
| Application | Chromogenic substrate for HRP in ELISA |
| Usage | - Add 100 μL of TMB solution per well and incubate for 5–30 min- - Add 100 μL of STOP solution per well to stop the reaction - Measure absorbance at 450 nm |
| Detection Sensitivity | Low picogram (pg) level |
| Volume | Approx. 20 × 96-well plates |
| Storage | 2–8°C, protected from light |
| Shelf Life | 1 year (unopened) |
Ordering Information
| Code No. | Description | Unit |
|---|---|---|
| 2332458 | WSE-7145 EzELISA TMB | 1 pk |
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