AE-1415 EzRunT

Purpose and Application

SDS-PAGE electrophoresis buffer for low molecular weight protein and polypeptide separation.

Features

Simple preparation: Dissolve in Distilled Water
Powder material: Easy keeping and carry
Simple and short time electrophoresis: Using with ATTO precast gel, p-PAGEL (for low molecular protein)

Data

Pattern data of protein samples

Gel: P-T16.5S (16.5%, 14-well gel)

Sample: WSE-7025 EzStandard LMW (M.W. marker) etc.

Running buffer: AE-1415 EzRunT (Tris, Tricine, SDS)

Electrophoresis system: AE-6530 mPAGE

Condition: Constant current 20 mA, 60 min

Staining: AE-1340 EzStain AQua (CBB staining)

ATTO Electrophoresis Buffer Selection Guide

Application	Running Buffer			Gel Buffer	Applicable Gels			Features
Target	Protein		DNA	DNA
Method	SDS-PAGE	Native-PAGE	DNA-PAGE	DNA-PAGE	Hand made Gels	ATTO Precast Gels	Agarose Gel
AE-1411 EzRun	〇	-	-	-	〇 Tris-based and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	-	A general-purpose Tris/Glycine/SDS running buffer compliant with the Laemmli method. Low cost; supplied as a powder for long-term storage.
AE-1412 EzRun C+	〇	-	-	-	〇 Tris-based and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	-	Contains reducing agents to suppress band broadening caused by re-oxidation during electrophoresis, enabling sharp band separation. Supplied as a powder.
AE-1415 EzRun T	〇	-	-	-	〇 Tricine-based polyacrylamide gels	〇 p-PAGEL, cp-PAGEL Neo	-	Tris/Tricine/SDS buffer for Tricine PAGE, suitable for separation of peptides and low–molecular-weight proteins.
WSE-7050 EzRun TAE	-	-	〇	〇			〇	Gel buffer / running buffer mainly used for DNA separation on agarose gels.
WSE-7051 EzRun TBE	-	-	〇	〇	〇 TBE-based, Tris-based, and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	〇	Gel buffer / running buffer mainly used for DNA separation. Can be used with both agarose gels and polyacrylamide gels.
WSE-7055 EzRun TG	-	〇	〇	〇	〇 Tris-based and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	-	Tris/Glycine buffer without SDS. Compatible with Native-PAGE for proteins and DNA. Enables BN-PAGE of proteins when supplemented with WSE-7067 EzBlueNative Additive.
WSE-7056 EzRun ClearNative	-	〇	-	-	〇 Tris-based and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	-	High-resolution Clear Native PAGE running buffer without SDS. Uses an anionic surfactant to minimize electrophoretic interference, allowing efficient separation of native (non-denatured) proteins.
WSE-7057 EzRun BlueNative	-	〇	-	-	〇 Tris-based and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	-	Blue Native PAGE running buffer without SDS. Supplied with EzRun BlueNative Additive. Coomassie Brilliant Blue (CBB) enables separation of native protein complexes without dissociation while minimizing electrophoretic interference.
WSE-7065 EzRun MOPS	〇		-	-	〇 Bis-Tris–based, imidazole-based, Tris-based, and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	-	Tris/MOPS/SDS buffer compatible with various polyacrylamide gels. Reduces protein mobility, improving resolution in the low–molecular-weight range. High electrophoretic speed enables rapid separation.
WSE-7066 EzRun MOPS non-SDS	-	〇	〇	〇	〇 Bis-Tris–based, imidazole-based, Tris-based, and Tris/Glycine–based polyacrylamide gels	〇 e-PAGEL, e-PAGEL HR, u-PAGEL H, c-PAGEL Neo	〇	Tris/MOPS buffer without SDS. Compatible with Native-PAGE for proteins and DNA. Enables BN-PAGE of proteins when supplemented with WSE-7067 EzBlueNative Additive.

Instruction Manual

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Specifications

	AE-1415 EzRunT
Major components	Tris, Tricine, SDS
Package	Powder/bag
Preparation	10X stock solution: Dissolve EzRunT 1 pack in 500 mL distilled water
Applicable amount	Max 5 L
Storage	1 year at room temperature (unopened)

Ordering Information

Code No.	Description	Unit
2332325	AE-1415 EzRunT	1 pk