Fractionation / Extraction Kit | Reagents

WSE-7424 EzProteoLysis Native

Purpose and Application

Extraction of native proteins (including membrane proteins) from animal and plant tissues as well as cultured cells without loss of activity
Sample preparation for Native PAGE/Enzyme activity assays/Sample preparation for electrophoresis/Sample preparation for Western blotting/Sample preparation for immunoprecipitation/Sample preparation for ELISA/Protein labeling

Features

Simple workflow: just mix the sample with the reagent solution, incubate, centrifuge, and collect the supernatant
Includes Protease Inhibitor and Phosphatase Inhibitor in the kit
Preserves protein structure and activity (e.g., enzymatic activity, fluorescence emission)
Maintains protein complexes and oligomeric structures

Data

Experimental Procedure

Proteins were extracted from chloroplasts (1 mg) using either 1% dodecyl maltoside (DDM) or WSE-7424 EzProteoLysis Native at volumes of 1, 2, 4, and 8 mL. WSE-7011 EzApply Native was then added to each extract at one-tenth volume. The extracted proteins were analyzed by Blue-Native PAGE using u-PAGEL H (3–10%) under WSE-7057 EzRun BlueNative conditions. EzProteoLysis Native exhibited protein extraction efficiency and electrophoretic patterns equivalent to those of 1% DDM.

Brochure

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Specifications

	WSE-7424 EzProteoLysis Native
Composition	1. EzProteoLysis Native: Glycerol, surfactant 2. Protease Inhibitor: 100× stock, Aprotinin, Pepstatin A, Leupeptin, DMSO 3. Phosphatase Inhibitor: 100× stock, Sodium fluoride, Sodium orthovanadate, Sodium β-glycerophosphate
Contents	1. EzProteoLysis Native: 30 mL 2. Protease Inhibitor: 0.3 mL 3. Phosphatase Inhibitor: 0.3 mL
Storage	1. EzProteoLysis Native: Refrigerated 2,3. Inhibitors: –20 °C
Shelf Life	1 year

Ordering Information

Code No.	Description	Unit
2332319	WSE-7424 EzProteoLysis Native	1 pk

WSE-7011 EzApply Native

WSE-7016 EzStandard Native

WSE-7423 EzBactYeastCrusher

WSE-7057 EzRun BlueNative