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WSE-7160 EzStain AQua MEM

WSE-7160 EzStain AQua MEM

Purpose and Application

  • Staining and destaining solution kit for detecting proteins on the membrane after blotting
  • Before the antibody reaction, carry out staining to confirm the blotting efficiency
  • The result can be used to normalize the target protein after the antibody reaction

Features

  • Can detect proteins on membrane
  • Staining and destaining in a short time
  • Bright blue band
  • Antigens and antibodies can be reacted after destaining
  • Support for normalization of total protein
  • Sensitivity is higher than Ponceau S
  • No acetic acid odor

Results

1. Membrane staining after blotting

The transferred membrane was cut in half, one stained by a conventional staining method (Ponceau S) (A), and the other stained and destained with WSE-7160 EzStain AQua MEM (B).
As for the sample, loaded a 3/4 serial dilution of Hela cell extract. WSE-7160 EzStain AQua MEM was detected at all concentrations.

2. Antibody reaction after staining

After the blotting, the membrane was cut in halt, one carried out an antibody reaction under normal operation to detect chemiluminescence (A).
And the other membrane was stained and destained with WSE-7160 EzStain AQua MEM, and an antibody reaction was performed to detect chemiluminescence (B).
It was shown that the antibody reaction does not affected by staining as the both membrane have the same sensitivity and patterns.

Operation Video

Instruction Manual

Specifications

 WSE-7160 EzStain AQua MEM
Major componentsStaining solution: Coomassie Brilliant Blue (CBB)
PackageWash (Pretreatment solution): 500 mL
Stain (Staining solution): 500 mL
De-Stain (Destaining solution): 500 mL
Bleach (Compelete destaining solution): 500 mL
PreparationWash: 2× dilute with methanol
De-Stain: 2× dilute with methanol
Bleach: 2× dilute with methanol
Applicable amount0.2~0.3 mL/cm²
20~25 sheets in mini size gel (80 × 95 mm)
StorageFor 1 year at room temperature

Ordering Information

Code No.DescriptionUnit
2332375WSE-7160 EzStain AQua MEM1 pk

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