WSE-7160 EzStain AQua MEM

Purpose and Application

Staining and destaining solution kit for detecting proteins on the membrane after blotting
Before the antibody reaction, carry out staining to confirm the blotting efficiency
The result can be used to normalize the target protein after the antibody reaction

Features

Can detect proteins on membrane
Staining and destaining in a short time
Bright blue band
Antigens and antibodies can be reacted after destaining
Support for normalization of total protein
Sensitivity is higher than Ponceau S
No acetic acid odor

Results

1. Membrane staining after blotting

The transferred membrane was cut in half, one stained by a conventional staining method (Ponceau S) (A), and the other stained and destained with WSE-7160 EzStain AQua MEM (B).
As for the sample, loaded a 3/4 serial dilution of Hela cell extract. WSE-7160 EzStain AQua MEM was detected at all concentrations.

2. Antibody reaction after staining

After the blotting, the membrane was cut in halt, one carried out an antibody reaction under normal operation to detect chemiluminescence (A).
And the other membrane was stained and destained with WSE-7160 EzStain AQua MEM, and an antibody reaction was performed to detect chemiluminescence (B).
It was shown that the antibody reaction does not affected by staining as the both membrane have the same sensitivity and patterns.

Operation Video

Instruction Manual

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Specifications

	WSE-7160 EzStain AQua MEM
Major components	Staining solution: Coomassie Brilliant Blue (CBB)
Package	Wash (Pretreatment solution): 500 mL Stain (Staining solution): 500 mL De-Stain (Destaining solution): 500 mL Bleach (Compelete destaining solution): 500 mL
Preparation	Wash: 2× dilute with methanol De-Stain: 2× dilute with methanol Bleach: 2× dilute with methanol
Applicable amount	0.2~0.3 mL/cm² 20~25 sheets in mini size gel (80 × 95 mm)
Storage	For 1 year at room temperature