WSE-7066 EzRun MOPS non-SDS

Purpose and Application

Electrophoresis buffer for nucleic acid separation using polyacrylamide gels (PAG)
Can also be used for agarose gel preparation and electrophoresis of nucleic acids
Suitable for protein electrophoresis in Native PAGE / BN PAGE (MOPS-based buffer)
Compatible with Bis-Tris gels for protein Native PAGE / BN PAGE

Features

Expands the separation range on the low-molecular side (from 10 bp) simply by replacing the buffer
Enables shorter electrophoresis time
Sterilized and stable for 1 year at room temperature
Convenient small-volume type for easy handling

Data

Differences in Electrophoresis Buffers — EzRun MOPS non-SDS vs. EzRun TG

The left figure compares the electrophoretic patterns and mobilities of DNA using ready-made gels of the same concentration as self-prepared TBE gels, with different electrophoresis buffers. It demonstrates that WSE-7066 EzRun MOPS non-SDS (left) is more suitable for separating low-molecular DNA, while WSE-7055 EzRun TG (right) is better suited for high-molecular DNA. By selecting the appropriate electrophoresis buffer, it is possible to adjust the separation range even when using polyacrylamide gels of the same concentration.

Band Mobility Depending on Gel Concentration and Electrophoresis Buffer

The left figure shows the separation of a 20 bp DNA ladder (left) and a 100 bp DNA ladder (right) using 5%, 10%, and 15% polyacrylamide gels (e-PAGEL). Electrophoresis buffers were WSE-7066 EzRun MOPS non-SDS (M) or WSE-7055 EzRun TG (G). After electrophoresis, gels were stained with WSE-7130 EzFluoroStain DNA, excited with CyanoView, and imaged using the WSE-5400 Printgraph Classic.

Results demonstrate that WSE-7066 EzRun MOPS non-SDS produces lower band mobility compared to WSE-7055 EzRun TG. Even with gels of the same concentration, switching to WSE-7066 expands the separation range on the low-molecular side.

Ideal for Separating 10–20 bp DNA Fragments!

The figure on the left shows DNA separation using a 15% polyacrylamide gel (EHR-T15L) after mixing a 20 bp DNA ladder and DNA fragments of the indicated sizes (two 22 bp bands differ in sequence) with WSE-7040 EzApply DNA. WSE-7066 EzRun MOPS non-SDS (left) or WSE-7055 EzRun TG (right) was used as the electrophoresis buffer. After electrophoresis, gels were stained with WSE-7130 EzFluoroStain DNA, excited with CyanoView, and imaged using the WSE-5400 Printgraph Classic.

These results demonstrate that WSE-7066 EzRun MOPS non-SDS allows clear separation of DNA fragments in the 10–20 bp range according to molecular weight, with even 1 bp differences distinctly resolved. In contrast, using WSE-7055 EzRun TG results in bands separated at nearly the same positions with only minor differences. By simply switching the buffer to WSE-7066 EzRun MOPS non-SDS while using standard ready-made gels, DNA fragments can be efficiently separated without denaturation by urea or other reagents.

Native PAGE of Proteins with EzRun MOPS non-SDS

The figure shows the separation of chloroplast-derived proteins (extracted with WSE-7424 EzProteoLysis Native) and E. coli–derived proteins (extracted with WSE-7423 EzBactYeast Crusher), mixed with WSE-7011 EzApply Native, and applied directly to ready-made gels (u-PAGEL H 3–10% and e-PAGEL 10%). Samples were separated by Blue Native PAGE (left) or Native PAGE (right). WSE-7066 EzRun MOPS non-SDS (left) or WSE-7055 EzRun TG (right) was used as the buffer, with WSE-7067 EzBlueNative Additive added at 1/100 volume only to the Blue Native PAGE cathode buffer.

Results indicate that WSE-7066 EzRun MOPS non-SDS provides lower band mobility than WSE-7055 EzRun TG. Moreover, adding WSE-7067 EzBlueNative Additive enables simple Blue Native PAGE, while also achieving clear band separation in Native PAGE.

Blue Native PAGE of Bis-Tris Gels with EzRun MOPS non-SDS

The figure shows the separation of chloroplast-derived proteins (extracted with WSE-7424 EzProteoLysis Native) and E. coli–derived proteins (extracted with WSE-7423 EzBactYeast Crusher), mixed with WSE-7011 EzApply Native, and applied directly to ready-made gels (commercial Bis-Tris 3–12% gels and ATTO u-PAGEL H 3–14% gels). WSE-7066 EzRun MOPS non-SDS was used as the buffer, with WSE-7067 EzBlueNative Additive added at 1/100 volume only to the cathode buffer.

These results demonstrate that WSE-7066 EzRun MOPS non-SDS can be used not only with Tris/Tris-Glycine gels but also with Bis-Tris gels without interfering with electrophoresis. Furthermore, adding WSE-7067 EzBlueNative Additive enables simple and convenient Blue Native PAGE.

Brochure

DOWNLOAD

Specifications

	WSE-7066 EzRun MOPS non-SDS
Components	Tris, MOPS, EDTA
Volume	250 mL (20× concentrate)
Usage	Dilute 20-fold with distilled water 220 mL × 22 runs (when using ATTO “Submerge Mini Electrophoresis System”) 450 mL × 11 runs (when using ATTO “Mini Slab Electrophoresis System”)
Storage / Shelf Life	1 year at room temperature, protected from light (unopened)

Ordering Information

Code No.	Description	Unit
2332306	WSE-7066 EzRun MOPS non-SDS	1 pk

WSE-7011 EzApply Native

WSE-7040 EzApply DNA

WSE-7423 EzBactYeastCrusher

WSE-7424 EzProteoLysis Native