WSE-7010 EzLabel FluoroNeo

Purpose and Application

Visualization of electrophoresis pattern with stain FREE

• Protein sample preparation for SDS-PAGE
• Fluorescent labeling for protein and polypeptide
• Protein electrophoresis (1D, 2D-PAGE)
• Immunoprecipitation, etc.

Features

• Immediate visualization protein bands after electrophoresis
  Protein fluorescence is emitted by BlueLED or UV excitation light
  [Ex: 330 (UV), 470 nm, Em: 530 nm]
• Stain FREE, Saving time, Unnecessary for liquid waste disposal
• Hardly to detect any differences in protein band mobility
• Observable the gel with Blue LED without removing glass plate gel holder
• Observed gel is available for western blotting and protein fluorescence is kept after transfer on membrane
• Observed gel is available for CBB staining and silver staining
• Kit includes RIPA Lysis Buffer (free amine buffer), MW marker

Western blotting with EzLabel FluoroNeo

Lane 1: Non-labeled MW marker
Lane 2: Labeled MW marker
Lane 3: Non-labeled HepG2 extract protein
Lane 4: Labeled HepG2 extract protein

After blotting, transferred proteins on membrane can be detected by excitation light.
Continuously process of western blotting reaction can be proceeded.

Operation Video

ATTO Molecular Weight Standard Selection Guide

The sample volume depends on the detection method. The number of uses is provided as a guideline.

Storage conditions: Store at −20 °C or below for up to one year (unopened). For more detailed product information, please refer to each product page.

¹ Staining with fluorescent dyes such as SYPRO Ruby or Oriole.

² Fluorescent detection may be possible depending on the fluorescence wavelength, filters, and antibodies used. When excited with red fluorescence, green and blue bands can be detected.

Brochure

Instruction Manual

Specifications

Ordering Information

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