WSE-7025 EzStandard LMW

Purpose and Application

Low molecular weight protein marker (about 1,000~29,000 Da) for SDS-PAGE

Features

sharp and clear band
Wide molecular weight range 1,000Da ~ 29,000Da
Store for one year and refrigerate for six months.

Data

Gel: p-PAGEL (ATTO precast gel for low molecular weight)

Sample: ★WSE-7025 EzStandard LMW

Running buffer: AE-1415 EzRunT (Tris-Tricine-SDS running buffer)

Electrophoresis system: WSE-1165 Mini-Slab

Condition: Constant voltage 175 V, 65 min

Staining: AE-1340 EzStain AQua (CBB staining)

Tips for Low Molecular Weight Protein Electrophoresis

Electrophoresis of proteins and polypeptides with a molecular weight of around 20,000 Da or less is difficult to separate in the usual SDS-PAGE (Laemmli method), so the electrophoresis is carried out with tris-tricin buffer (AE-1415 EzRunT).

Gels and electrophoresis buffers are used exclusively for low molecular weight protein separation. The minimum time for the migration is about 65 minutes, and the migration is completed faster than before.

Low molecular weight proteins and polypeptides can spread if it is not fixed immediately after migration or not stained for a short period of time. CBB staining with AE-1340 EzStain AQua can be stained and destained for a short time using the microwave oven method.

Instruction Manual

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Specifications

	WSE-7025 EzStandard LMW
Components	6 protein and polypeptides 1000, 3500, 6500, 14300, 17000, 29000 Da
Package	Peptide stock (20x) 0.1 mL Sample buffer (2x) 10 mL DTT 150 mg
Preparation	Mix Peptide stock, Sample buffer, and DTT and heat at 70℃ for 5-10 minutes before use
Applicable amount	approximately 400 to 1,000 times (2~5 μL/well)
Storage	1 year at -20℃, 6 months at 4℃ (after preparation)