Purpose and Application
- Low molecular weight protein marker (about 1,000~29,000 Da) for SDS-PAGE
- sharp and clear band
- Wide molecular weight range 1,000Da ~ 29,000Da
- Store for one year and refrigerate for six months.
Gel: p-PAGEL (ATTO precast gel for low molecular weight)
Sample: ★WSE-7025 EzStandard LMW
Running buffer: AE-1415 EzRunT (Tris-Tricine-SDS running buffer)
Electrophoresis system: WSE-1165 Mini-Slab
Condition: Constant voltage 175 V, 65 min
Staining: AE-1340 EzStain AQua (CBB staining)
Tips for Low Molecular Weight Protein Electrophoresis
Electrophoresis of proteins and polypeptides with a molecular weight of around 20,000 Da or less is difficult to separate in the usual SDS-PAGE (Laemmli method), so the electrophoresis is carried out with tris-tricin buffer (AE-1415 EzRunT).
Gels and electrophoresis buffers are used exclusively for low molecular weight protein separation. The minimum time for the migration is about 65 minutes, and the migration is completed faster than before.
Low molecular weight proteins and polypeptides can spread if it is not fixed immediately after migration or not stained for a short period of time. CBB staining with AE-1340 EzStain AQua can be stained and destained for a short time using the microwave oven method.
|WSE-7025 EzStandard LMW|
|Components||6 protein and polypeptides
1000, 3500, 6500, 14300, 17000, 29000 Da
|Package||Peptide stock (20x) 0.1 mL
Sample buffer (2x) 10 mL
DTT 150 mg
|Preparation||Mix Peptide stock, Sample buffer, and DTT and heat at 70℃ for 5-10 minutes before use|
|Applicable amount||approximately 400 to 1,000 times (2~5 μL/well)|
|Storage||1 year at -20℃, 6 months at 4℃ (after preparation)|
|2332348||WSE-7025 EzStandard LMW||1 set|