cp-PAGEL Neo

Purpose and Application

“cp-PAGEL Neo” is a high-performance, ready-to-use polyacrylamide gel (glass plate size: 60 mm × 60 mm) for the separation of low molecular weight proteins.

Features

Compact-sized gel for Tricine-PAGE (6 cm × 6 cm), ideal for separating low molecular weight proteins
As little as 15 minutes under high-voltage (rapid) conditions — electrophoresis can be completed in about 60 minutes under standard conditions
Expiration date : Can be stored in a refrigerator for up to 1 year from the date of manufacture
Requires only small amounts of samples and reagents
Precast gel with consistent quality and high reproducibility
Compact design ensures easy handling—simple to set up and remove
Compatible electrophoresis apparatus: ATTO Compact Gel Size Electrophoresis System (WSE-1030 Compact PAGE Neo, WSE-1040 Compact PAGE Neo)

Description

Electrophoretic image

Experimental Conditions

Gel: cp-PAGEL Neo (CPN-16.5S)
Electrophoresis buffer: 1× EzRunT (Tris-Tricine-SDS)
Electrophoresis system: Compact PAGE Neo
Staining method: EzStain AQua (rapid staining protocol)

Post-electrophoresis staining

After separation with cp-PAGEL Neo, the gel was stained either with EzStain AQua (a rapid staining method) or with CBB staining solution (0.25% CBB, 40% methanol, 10% acetic acid) following fixation.
Bands at 1.0, 2.5, and 3.5 kDa—highlighted by the dotted area—were detected only with EzStain AQua.
To effectively visualize low molecular weight proteins, it is essential to perform staining and destaining as quickly as possible.
Since EzStain AQua is alcohol-free, it minimizes gel deformation due to dehydration. Additionally, its short staining time reduces protein diffusion, making it highly suitable for detecting low molecular weight proteins.

* Gel staining method

1. Standard Method

Immerse the gel in EzStain AQua and gently shake for at least 3 hours.
Replace the solution with distilled water and destain until the background becomes transparent.

2. Rapid Method

Immerse the gel in EzStain AQua, then heat in a microwave (600 W) for 1 minute.
Gently shake for 10 to 60 minutes to allow staining.
Replace with distilled water, heat in the microwave again, and then shake at room temperature for 30 to 60 minutes to destain until the background becomes transparent.

Specifications

	CPN-16.5S cp-PAGEL Neo 16.5% 15-well
Usage	Polyacrylamide gel electrophoresis of polypeptides and proteins (Tricine PAGE)
Glass plate size	76 (W) x 70 (H) mm
Gel size	60 (W) x 60 (H) mm, thickness 1 mm
Gel/buffer composition	Polyacrylamide
Gel Concentration	16.5 %
Separation range	1000-75000 Da
Sample wells	15-wells (width 2 mm) maximum 9 μL/well
Storage	1 years when stored in a refrigerator (NOT to be frozen)
Package	10 plates/box

* Gels do NOT contain SDS.
* For low molecular weight protein separation, use Tris-Tricine-SDS buffer (AE-1415 EzRunT).
* cp-PAGEL Neo is a ready-to-use gel designed for Tricine-PAGE. For SDS-PAGE or Native PAGE, use c-PAGEL Neo in combination with AE-1410 EzRun (SDS-PAGE buffer) or WSE-7055 EzRunTG (SDS-free buffer), respectively.
* We recommend storing the gel at a temperature between 5°C and 10°C. If it is placed near the cold air outlet of the refrigerator, the temperature may drop below 4°C, which can cause the gel to freeze and become unusable.