WSE-7056 EzRun ClearNative

Purpose and Application

• Buffer for HR-CN-PAGE (High-Resolution Clear Native PAGE)

Features

• A set of 5× concentrated cathode and anode electrophoresis buffers

• Simply dilute 5-fold with distilled water before use

• Does not inhibit enzyme activity or protein functionality

• Enables HR-CN-PAGE with SDS-free Tris-Glycine polyacrylamide gels

Data

Protocol

The required buffer volume may vary depending on the electrophoresis tank. Please refer to the table below.

Comparison with conventional Bis-Tris gel methods

The figure above shows the results of CN-PAGE performed with a self-prepared Bis-Tris gel (according to Schägger et al., 2008) compared with the results of HR-CN-PAGE carried out using the Tris-Glycine gel UH-T310. HR-CN-PAGE was originally developed based on Bis-Tris gels and was not suitable for Tris-Glycine gels. EzRun ClearNative enables HR-CN-PAGE to be performed on Tris-Glycine polyacrylamide gels while maintaining correlation with the electrophoresis patterns obtained using Bis-Tris gels.

Protein activity staining

The figure above shows the results of β-galactosidase activity staining after electrophoretic separation using HR-CN-PAGE, conventional Native PAGE, and SDS-PAGE, captured with the Printgraph Classic. HR-CN-PAGE, like conventional Native PAGE, allows detection without inhibiting enzyme activity. In contrast, SDS-PAGE does not produce detectable bands because SDS affects enzyme activity.

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Specifications

Ordering Information

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