AE-1340 EzStain AQua

Purpose and Application

Detection of protein

Features

Applicable to PAGE of proteins
Absence of organic solvents and acetic acid reduces concern about waste liquid and bad smell
Ready to use. Easily prepared only by pouring to the tray
Staining time of only 30 to 120 minutes for detection
Discoloration with distilled water results in nearly transparent background
Sensitivity is up to several ng of protein per band
A sample is available. Please request the sample through the “Contact Us” menu at the top.

Data

Sample: human transferrin 400 ng/lane (a series of two-fold dilution)

Common CBB stains after staining in 10 minutes, the band cannot see, but EzStain AQua can verify the band.

Detect limit of common CBB is 25 ng/lane
Detect limit of EzStain AQua is 6.2 ng/lane

Time saving, More sensitive staining solution, EzStain AQua

ATTO Protein / Nucleic Acid Staining Selection Guide

	AE-1340 EzStain AQua	AE-1360 EzStain Silver	AE-1310 EzStain Reverse	WSE-7160 EzStain AQua MEM	WSE-7130 EzFluoroStain DNA	WSE-7135 EzPreStain DNA&RNA
Application	Protein staining	Protein / Nucleic acid (DNA/RNA) staining	Protein staining	Blotting membrane staining	Nucleic acid (DNA) staining (ssDNA not stainable)	Nucleic acid (DNA/RNA) staining
Gel	Polyacrylamide gel	Agarose gel / Polyacrylamide gel	Polyacrylamide gel	-	Agarose gel / Polyacrylamide gel	Agarose gel / Polyacrylamide gel
Detection	Colorimetric (blue staining) Visualized under visible light	Colorimetric (brown staining) Imaged under bright-field (High background when TAE or EDTA-containing buffers are used)	Colorimetric (Areas other than bands appear white) Imaged under bright-field on a black sheet	Colorimetric (blue staining) Visualized under visible light	Fluorescence Ex: 270/370/497 nm, Em: 522 nm UV or Cyan LED excitation Orange filter / YA-3	Fluorescence Ex: 250/482 nm, Em: 509 nm UV or Cyan LED excitation Orange filter / YA-3
Features	Ready-to-use High-sensitivity CBB staining (10 ng protein) Low background Methanol- & acetic acid-free Staining completed within 30 min Destaining with water only	Easy-to-use High-sensitivity staining (1 ng protein, 10 pg DNA) Aldehyde-free Suitable for mass spectrometry (MS) analysis Staining completed within 55 min	Easy-to-use High-sensitivity negative staining (2 ng protein) Non-staining protein detection method (White background) Suitable for protein extraction from gels Staining completed within 25 min	Easy-to-use Rapid detection (1 min staining, 5 min destaining) Antibody reactions possible after staining Suitable for total protein normalization Higher sensitivity than Ponceau S	Easy-to-use High-sensitivity detection with low background Safe under UV / Blue(Cyan) LED excitation Lower mutagenicity than EtBr (Ames test) No destaining required	Easy-to-use Fluorescent detection of single- and double-stranded DNA/RNA Pre-staining possible (add to gel preparation or running buffer) Post-staining also possible
Volume	1 / 5 / 10 L (3 sizes available)	① S-1: Sodium thiosulfate ② S-2: Silver nitrate ③ S-3: Sodium hydroxide ④ S-4: Sodium thiosulfate, formaldehyde 50 mL each	① R-1: Boric acid, SDS ② R-2: Zinc sulfate 500 mL each	① Wash solution ② Stain solution ③ De-stain solution ④ Bleach (complete de-staining) 500 mL each	500 µL (10,000× concentrate)	500 µL (10,000× concentrate)
Preparation	Not required	Dilute each reagent 100-fold with distilled water	distilled water Mix 10 mL of each solution with 50 mL of distilled water	Except for the stain solution, mix with methanol in equal volume (Methanol not included)	Dilute 10,000-fold with TAE or TBE	Add 1/10,000 volume during gel preparation Dilute 10,000-fold with TAE or TBE
Usage	50 mL per mini-gel 20–25 mini-gels	Approx. 50 mini-gels	Approx. 50 mini-gels	20–25 mini-gels	-	-
Storage	Room temperature, 1 year	Refrigerated, protected from light, 2 years	Room temperature, protected from light, 2 years	Room temperature, 1 year	−20 °C, protected from light, 1 year	−20 °C, protected from light, 1 year

Operation Video

Instruction Manual

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Specifications

	AE-1340 EzStain AQua	AE-1340L EzStain AQua	AE-1340LL EzStain AQua
Major components	Coomassie brilliant blue (CBB), acid, and stabilizing reagent
Preparation	Ready to use
Applicable amount	Approximately 20 sheets of mini-gel	Approximately 100 sheets of mini-gel	Approximately 200 sheets of mini-gel
Storage	1 year at room temperature (unopened)
Package	1 L/bottle	5 L/pk	10 L/pk

Ordering Information

Code No.	Description	Unit
2332370	AE-1340 EzStain AQua, 1 L	1 pk
2332371	AE-1340L EzStain AQua, 5 L	1 pk
2332372	AE-1340LL EzStain AQua, 10 L	1 pk