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c-PAGEL

Purpose and Application

  • Separation and detection of proteins (C type)
  • Second step of 2D electrophoresis (CD type)
  • SDS-PAGE, Native-PAGE
  • Detection of PCR products, separation of DNA
  • Screening
  • Experimental training for students or researchers

Features

  • Compact size (60 mm x 60 mm)
  • Fast electrophoresis run time as 30 min
  • Even with small amount of samples and reagents, gives good separation
  • Offers stability and reproducibility
  • Glass plates with good thermal stability and uniformity employed
  • Easy to set on to the apparatus and take off the gel from the glass plate
  • Varieties of gels are available, including wide range of gel percentage, wells, gels for 2D electrophoresis also available
  • Compatible electrophoresis apparatus: ATTO Compact Gel Size Electrophoresis System (WSE-1010 cPAGE Ace, WSE-1025 cPAGE Ace Twin)

Separation Range

C15S is discontinued. Please use cp-PAGEL.

Data

Pattern data of protein samples

Gel: C10L (10%, 12-well gel)

Sample: M.W. marker etc.

Running buffer: AE-1410 EzRun (25 mM Tris, 192 mM Glycine, 0.1% SDS)

Electrophoresis system: WSE-1025 cPAGE Ace Twin

Condition: Constant current 21 mA, 30 min

Staining: AE-1340 EzStain AQua (CBB staining)

Pattern data of DNA samples

Gel: C7.5L (7.5%, 12-well gel)

Sample: DNA ladder etc.

Running buffer: WSE-7055 EzRunTG (25 mM Tris, 192 mM Glycine)

Electrophoresis system: WSE-1025 cPAGE Ace Twin

Condition: Constant current 21 mA, 80 min

Staining: EtBr staining

Specifications

 CXL c-PAGEL 12-wellsCDXL c-PAGEL for 2D electrophoresis
UsagePolyacrylamide gel electrophoresis of protein or nucleic acid (SDS-PAGE, native PAGE etc)
Glass plate size76 (W) x 70 (H) mm
Gel size60 (W) x 60 (H) mm, thickness 0.75 mm
Gel/buffer compositionPolyacrylamide, Tris-HCl buffer
Sample wells12-wells (width 2.9 mm)no well
sample loading volumemaximum 8 μL/wellgel width 55 mm (depending on the first step IEF gel length)
Storage6 months when stored in a refrigerator (NOT to be frozen)
Package10 plates/box

* Gels do NOT contain SDS. Electrophoresis with buffer solution containing 24mM Tris + 192mM Glycine + 0.1% SDS (AE-1410 EzRun) is Laemmli method, and one without SDS (WSE-7055 EzRun TG) is Ornstein-Davis method.

* Use buffer solution containing 25mM Tris and 192mM Glycine or our product, [WSE-7055 EzRunTG] for DNA electrophoresis.

Ordering Information

Code No.DescriptionUnit
2331610C7.5L c-PAGEL 7.5% 12-well 10 gels1 pk
2331620C10L c-PAGEL 10% 12-well 10 gels1 pk
2331630C12.5L c-PAGEL 12.5% 12-well 10 gels1 pk
2331640C15L c-PAGEL 15% 12-well 10 gels1 pk
2331600C520L c-PAGEL 5~20% 12-well 10 gels1 pk
2331650CD10L c-PAGEL 10% for 2-DE 10 gels1 pk
2331660CD12.5L c-PAGEL 12.5% for 2-DE 10 gels1 pk
2331670CD520L c-PAGEL 5~20% for 2-DE 10 gels1 pk

Related products

WSE-1010 cPAGE Ace
WSE-1010 cPAGE Ace
WSE-1025 cPAGE Ace Twin
WSE-1025 cPAGE Ace Twin
c-PAGEL HR
c-PAGEL HR
cp-PAGEL
cp-PAGEL
AE-1410 EzRun
AE-1410 EzRun
WSE-7055 EzRun TG
WSE-7055 EzRun TG