c-PAGEL
Purpose and Application
- Separation and detection of proteins (C type)
- Second step of 2D electrophoresis (CD type)
- SDS-PAGE, Native-PAGE
- Detection of PCR products, separation of DNA
- Screening
- Experimental training for students or researchers
Features
- Compact size (60 mm x 60 mm)
- Fast electrophoresis run time as 30 min
- Even with small amount of samples and reagents, gives good separation
- Offers stability and reproducibility
- Glass plates with good thermal stability and uniformity employed
- Easy to set on to the apparatus and take off the gel from the glass plate
- Varieties of gels are available, including wide range of gel percentage, wells, gels for 2D electrophoresis also available
- Compatible electrophoresis apparatus: ATTO Compact Gel Size Electrophoresis System (WSE-1010 cPAGE Ace, WSE-1025 cPAGE Ace Twin)
Separation Range
| Type | Gel concentration | Separation range | ||
| 12-wells | no well (for 2-D) | Protein (Da) | DNA (bp) | |
| C7.5L | - | 7.5% | 40,000 ~ 400,000 | 250 ~ 3,000 |
| C10L | CD10L | 10% | 25,000 ~ 300,000 | 150 ~ 2,000 |
| C12.5L | CD12.5L | 12.5% | 10,000 ~ 250,000 | 70 ~ 1,800 |
| C15L | - | 15% | 5,000 ~ 200,000 | 50 ~ 1,500 |
| C520L | CD520L | 5 ~ 20% | 5,000 ~ 400,000 | 50 ~ 3,000 |
For low molecular weight protein, please use cp-PAGEL.
Data
Pattern data of protein samples
Gel: C10L (10%, 12-well gel)
Sample: M.W. marker etc.
Running buffer: AE-1410 EzRun (25 mM Tris, 192 mM Glycine, 0.1% SDS)
Electrophoresis system: WSE-1025 cPAGE Ace Twin
Condition: Constant current 21 mA, 30 min
Staining: AE-1340 EzStain AQua (CBB staining)
Pattern data of DNA samples
Gel: C7.5L (7.5%, 12-well gel)
Sample: DNA ladder etc.
Running buffer: WSE-7055 EzRunTG (25 mM Tris, 192 mM Glycine)
Electrophoresis system: WSE-1025 cPAGE Ace Twin
Condition: Constant current 21 mA, 80 min
Staining: EtBr staining
Specifications
| CXL c-PAGEL 12-wells | CDXL c-PAGEL for 2D electrophoresis | |
|---|---|---|
| Usage | Polyacrylamide gel electrophoresis of protein or nucleic acid (SDS-PAGE, native PAGE etc) | |
| Glass plate size | 76 (W) x 70 (H) mm | |
| Gel size | 60 (W) x 60 (H) mm, thickness 0.75 mm | |
| Gel/buffer composition | Polyacrylamide, Tris-HCl buffer | |
| Sample wells | 12-wells (width 2.9 mm) | no well |
 |  |
|
| sample loading volume | maximum 8 μL/well | gel width 55 mm (depending on the first step IEF gel length) |
| Storage | 6 months when stored in a refrigerator (NOT to be frozen) | |
| Package | 10 plates/box | |
* Gels do NOT contain SDS. Electrophoresis with buffer solution containing 24mM Tris + 192mM Glycine + 0.1% SDS (AE-1410 EzRun) is Laemmli method, and one without SDS (WSE-7055 EzRun TG) is Ornstein-Davis method.
* Use buffer solution containing 25mM Tris and 192mM Glycine or our product, [WSE-7055 EzRunTG] for DNA electrophoresis.
Ordering Information
| Code No. | Description | Unit |
|---|---|---|
| 2331610 | C7.5L c-PAGEL 7.5% 12-well 10 gels | 1 pk |
| 2331620 | C10L c-PAGEL 10% 12-well 10 gels | 1 pk |
| 2331630 | C12.5L c-PAGEL 12.5% 12-well 10 gels | 1 pk |
| 2331640 | C15L c-PAGEL 15% 12-well 10 gels | 1 pk |
| 2331600 | C520L c-PAGEL 5~20% 12-well 10 gels | 1 pk |
| 2331650 | CD10L c-PAGEL 10% for 2-DE 10 gels | 1 pk |
| 2331660 | CD12.5L c-PAGEL 12.5% for 2-DE 10 gels | 1 pk |
| 2331670 | CD520L c-PAGEL 5~20% for 2-DE 10 gels | 1 pk |
Related products
WSE-1010 cPAGE Ace
WSE-1025 cPAGE Ace Twin
c-PAGEL HR
cp-PAGEL
AE-1410 EzRun
