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Purpose and Application

Best for Low molecular weight proteins and polypeptides separation

  • Separation and detection of proteins and polypeptides
  • Screening
  • Experimental training for students or researchers


  • Wide Separation  Range of 1,000-75,000Da
  • sharp band
  • The minimum time for migration is 15 minutes!Combine fast migration with clean migration patterns
  • A year of shelf life
  • Stable in quality and reproducibility
  • Adopting glass plates with excellent thermostatic efficiency
  • Easy to set up and remove gel
  • Compatible electrophoresis apparatus: ATTO Compact Gel Size Electrophoresis System (WSE-1010 cPAGE Ace, WSE-1025 cPAGE Ace Twin)


Pattern data of protein samples

Gel: CP16.5S (16.5%, 15-well gel)

Sample: ① Chicken muscle extract
WSE-7025 EzStandard LMW
WSE-7020 EzProtein Ladder
AE-1440 EzStandard
⑤ HeLa Cell Extract

Running buffer: 2X AE-1415 EzRunT (Tris, Tricine, SDS)

Electrophoresis system: WSE-1010 cPAGE Ace

Condition: High mode (24W), 15 min

Staining: AE-1340 EzStain AQua (CBB staining)

Tips for Low Molecular Weight Protein Electrophoresis

Electrophoresis of proteins and polypeptides with a molecular weight of around 20,000 Da or less is difficult to separate in the usual SDS-PAGE (Laemmli method), so the electrophoresis is carried out with tris-tricin buffer (AE-1415 EzRunT).

Gels and electrophoresis buffers are used exclusively for low molecular weight protein separation. The minimum time for the migration is about 65 minutes, and the migration is completed faster than before.

Low molecular weight proteins and polypeptides can spread if it is not fixed immediately after migration or not stained for a short period of time. CBB staining with AE-1340 EzStain AQua can be stained and destained for a short time using the microwave oven method.


 CP16.5S cp-PAGEL (15-wells)
UsagePolyacrylamide gel electrophoresis of protein or polypeptide (SDS-PAGE etc)
Glass plate size76 (W) x 70 (H) mm
Gel size60 (W) x 60 (H) mm, thickness 0.75 mm
Gel/buffer compositionPolyacrylamide 16.5%, Tris-Tricine buffer
Separation range1,000~75,000 Da
Sample wells15-wells (width 2 mm)
sample loading volumemaximum 7 μL/well
Storage1 year when stored in a refrigerator (NOT to be frozen)
Package10 plates/box

Ordering Information

Code No.DescriptionUnit
2331695CP16.5S cp-PAGEL 15-well 10 gels1 pk

Related products

AE-1430 EzApply
AE-1430 EzApply
AE-1415 EzRunT
AE-1415 EzRunT
WSE-7025 EzStandard LMW
WSE-7025 EzStandard LMW
AE-1340 EzStain AQua
AE-1340 EzStain AQua
WSE-1010 cPAGE Ace
WSE-1010 cPAGE Ace
WSE-1025 cPAGE Ace Twin
WSE-1025 cPAGE Ace Twin