c-PAGEL HR

Purpose and Application

Separation and detection of proteins (CHR type)
Second step of 2D electrophoresis (CHRD type)
SDS-PAGE, Native-PAGE
Detection of PCR products, separation of DNA
Screening
Experimental training for students or researchers

Features

High-speed migration is possible: shortest migration time is 10 min. (standard 30 min)
Small quantities of samples and reagents are also OK.
Expiration date of use: 1 year
Minimizing smiling
Stable quality, High reproducibility
Glass plates with good thermal stability
Easy to set on the apparatus and take off the gel from the glass plate
Varieties of gels are available, including wide range of gel percentage and wells
Compatible electrophoresis apparatus: ATTO Compact Gel Size Electrophoresis System (WSE-1010 cPAGE Ace, WSE-1025 cPAGE Ace Twin)

Separation Range

Type		Gel concentration	Separation range
15-wells	no well (for 2-D)	Gel concentration	Protein (Da)	DNA (bp)
CHR7.5L	-	7.5%	40,000 ~ 400,000	250 ~ 3,000
CHR10L	-	10%	20,000 ~ 300,000	100 ~ 2,000
CHR12.5L	-	12.5%	10,000 ~ 250,000	70 ~ 1,800
CHR15L	-	15%	5,000 ~ 200,000	50 ~ 1,500
CHR520L	CHRD520L	5 ~ 20%	3,500 ~ 400,000	30 ~ 2,500

Data

Pattern data of protein samples

Gel: CHR520L (5-20%, 15-well gel)

Sample: AE-1440 EzStandard, WSE-7020 EzProtein Ladder etc.

Running buffer: AE-1410 EzRun (25 mM Tris, 192 mM Glycine, 0.1% SDS)

Electrophoresis system: WSE-1010 cPAGE Ace

Condition: High mode (24W), 10 min

Staining: AE-1340 EzStain AQua (CBB staining)

Specifications

	CHRXL c-PAGEL HR 15-wells	CHRDXL c-PAGEL HR for 2D electrophoresis
Usage	Polyacrylamide gel electrophoresis of protein or nucleic acid (SDS-PAGE, native PAGE etc)
Glass plate size	76 (W) x 70 (H) mm
Gel size	60 (W) x 60 (H) mm, thickness 0.75 mm
Gel/buffer composition	Polyacrylamide, Tris-HCl buffer
Sample wells	15-wells (width 2 mm)	no well
sample loading volume	maximum 7 μL/well	gel width 55 mm (depending on the first step IEF gel length)
Storage	1 year when stored in a refrigerator (NOT to be frozen)
Package	10 plates/box

* Gels do NOT contain SDS. Electrophoresis with buffer solution containing 24mM Tris + 192mM Glycine + 0.1% SDS (AE-1410 EzRun) is Laemmli method, and one without SDS (WSE-7055 EzRun TG) is Ornstein-Davis method.

* We recommend buffer [WSE-7065 EzRunMOPS] for high-speed migration.

* Use buffer solution containing 25mM Tris and 192mM Glycine or our product, [WSE-7055 EzRunTG] for DNA electrophoresis.

* The way of making [e-PAGEL HR] is different from that of [e-PAGEL] so that the migration pattern may not be same.

Ordering Information

Code No.	Description	Unit
2331615	CHR7.5L c-PAGEL HR 7.5% 15-well 10 gels	1 pk
2331625	CHR10L c-PAGEL HR 10% 15-well 10 gels	1 pk
2331635	CHR12.5L c-PAGEL HR 12.5% 15-well 10 gels	1 pk
2331645	CHR15L c-PAGEL HR 15% 15-well 10 gels	1 pk
2331605	CHR520L c-PAGEL HR 5~20% 15-well 10 gels	1 pk
2331675	CHRD520L c-PAGEL HR 5~20% for 2-DE 10 gels	1 pk