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c-PAGEL HR

Purpose and Application

  • Separation and detection of proteins (CHR type)
  • Second step of 2D electrophoresis (CHRD type)
  • SDS-PAGE, Native-PAGE
  • Detection of PCR products, separation of DNA
  • Screening
  • Experimental training for students or researchers

Features

  • High-speed migration is possible: shortest migration time is 10 min. (standard 30 min)
  • Small quantities of samples and reagents are also OK.
  • Expiration date of use: 1 year
  • Minimizing smiling
  • Stable quality, High reproducibility
  • Glass plates with good thermal stability
  • Easy to set on the apparatus and take off the gel from the glass plate
  • Varieties of gels are available, including wide range of gel percentage and wells
  • Compatible electrophoresis apparatus: ATTO Compact Gel Size Electrophoresis System (WSE-1010 cPAGE Ace, WSE-1025 cPAGE Ace Twin)

Separation Range

Data

Pattern data of protein samples

Gel: CHR520L (5-20%, 15-well gel)

Sample: AE-1440 EzStandard, WSE-7020 EzProtein Ladder etc.

Running buffer: AE-1410 EzRun (25 mM Tris, 192 mM Glycine, 0.1% SDS)

Electrophoresis system: WSE-1010 cPAGE Ace

Condition: High mode (24W), 10 min

Staining: AE-1340 EzStain AQua (CBB staining)

Specifications

 CHRXL c-PAGEL HR 15-wellsCHRDXL c-PAGEL HR for 2D electrophoresis
UsagePolyacrylamide gel electrophoresis of protein or nucleic acid (SDS-PAGE, native PAGE etc)
Glass plate size76 (W) x 70 (H) mm
Gel size60 (W) x 60 (H) mm, thickness 0.75 mm
Gel/buffer compositionPolyacrylamide, Tris-HCl buffer
Sample wells15-wells (width 2 mm)no well
sample loading volumemaximum 7 μL/wellgel width 55 mm (depending on the first step IEF gel length)
Storage1 year when stored in a refrigerator (NOT to be frozen)
Package10 plates/box

* Gels do NOT contain SDS. Electrophoresis with buffer solution containing 24mM Tris + 192mM Glycine + 0.1% SDS (AE-1410 EzRun) is Laemmli method, and one without SDS (WSE-7055 EzRun TG) is Ornstein-Davis method.

* We recommend buffer [WSE-7065 EzRunMOPS] for high-speed migration.

* Use buffer solution containing 25mM Tris and 192mM Glycine or our product, [WSE-7055 EzRunTG] for DNA electrophoresis.

* The way of making [e-PAGEL HR] is different from that of [e-PAGEL] so that the migration pattern may not be same.

Ordering Information

Code No.DescriptionUnit
2331615CHR7.5L c-PAGEL HR 7.5% 15-well 10 gels1 pk
2331625CHR10L c-PAGEL HR 10% 15-well 10 gels1 pk
2331635CHR12.5L c-PAGEL HR 12.5% 15-well 10 gels1 pk
2331645CHR15L c-PAGEL HR 15% 15-well 10 gels1 pk
2331605CHR520L c-PAGEL HR 5~20% 15-well 10 gels1 pk
2331675CHRD520L c-PAGEL HR 5~20% for 2-DE 10 gels1 pk

Related products

WSE-1010 cPAGE Ace
WSE-1010 cPAGE Ace
WSE-1025 cPAGE Ace Twin
WSE-1025 cPAGE Ace Twin
AE-1410 EzRun
AE-1410 EzRun
WSE-7065 EzRun MOPS
WSE-7065 EzRun MOPS
WSE-7055 EzRun TG
WSE-7055 EzRun TG
c-PAGEL
c-PAGEL
cp-PAGEL
cp-PAGEL