c-PAGEL HR

Purpose and Application
- Separation and detection of proteins (CHR type)
- Second step of 2D electrophoresis (CHRD type)
- SDS-PAGE, Native-PAGE
- Detection of PCR products, separation of DNA
- Screening
- Experimental training for students or researchers
Features
- High-speed migration is possible: shortest migration time is 10 min. (standard 30 min)
- Small quantities of samples and reagents are also OK.
- Expiration date of use: 1 year
- Minimizing smiling
- Stable quality, High reproducibility
- Glass plates with good thermal stability
- Easy to set on the apparatus and take off the gel from the glass plate
- Varieties of gels are available, including wide range of gel percentage and wells
- Compatible electrophoresis apparatus: ATTO Compact Gel Size Electrophoresis System (WSE-1010 cPAGE Ace, WSE-1025 cPAGE Ace Twin)
Separation Range

Data

Pattern data of protein samples
Gel: CHR520L (5-20%, 15-well gel)
Sample: AE-1440 EzStandard, WSE-7020 EzProtein Ladder etc.
Running buffer: AE-1410 EzRun (25 mM Tris, 192 mM Glycine, 0.1% SDS)
Electrophoresis system: WSE-1010 cPAGE Ace
Condition: High mode (24W), 10 min
Staining: AE-1340 EzStain AQua (CBB staining)
Specifications
CHRXL c-PAGEL HR 15-wells | CHRDXL c-PAGEL HR for 2D electrophoresis | |
---|---|---|
Usage | Polyacrylamide gel electrophoresis of protein or nucleic acid (SDS-PAGE, native PAGE etc) | |
Glass plate size | 76 (W) x 70 (H) mm | |
Gel size | 60 (W) x 60 (H) mm, thickness 0.75 mm | |
Gel/buffer composition | Polyacrylamide, Tris-HCl buffer | |
Sample wells | 15-wells (width 2 mm) | no well |
sample loading volume | maximum 7 μL/well | gel width 55 mm (depending on the first step IEF gel length) |
Storage | 1 year when stored in a refrigerator (NOT to be frozen) | |
Package | 10 plates/box |
* Gels do NOT contain SDS. Electrophoresis with buffer solution containing 24mM Tris + 192mM Glycine + 0.1% SDS (AE-1410 EzRun) is Laemmli method, and one without SDS (WSE-7055 EzRun TG) is Ornstein-Davis method.
* We recommend buffer [WSE-7065 EzRunMOPS] for high-speed migration.
* Use buffer solution containing 25mM Tris and 192mM Glycine or our product, [WSE-7055 EzRunTG] for DNA electrophoresis.
* The way of making [e-PAGEL HR] is different from that of [e-PAGEL] so that the migration pattern may not be same.
Ordering Information
Code No. | Description | Unit |
---|---|---|
2331615 | CHR7.5L c-PAGEL HR 7.5% 15-well 10 gels | 1 pk |
2331625 | CHR10L c-PAGEL HR 10% 15-well 10 gels | 1 pk |
2331635 | CHR12.5L c-PAGEL HR 12.5% 15-well 10 gels | 1 pk |
2331645 | CHR15L c-PAGEL HR 15% 15-well 10 gels | 1 pk |
2331605 | CHR520L c-PAGEL HR 5~20% 15-well 10 gels | 1 pk |
2331675 | CHRD520L c-PAGEL HR 5~20% for 2-DE 10 gels | 1 pk |