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WSL-1800 CytoWatcher

WSL-1800 CytoWatcher

Purpose and Application

  • For bright field cell imaging with low magnification.
  • Compact size, no stress to take space.
  • Usable in a CO₂ incubator, for time-lapse imaging in culture condition.
  • Equipped with 5M pixels color CMOS camera.
  • Multiple samples can be taken by connecting multiple units.
  • A model for fluorescent imaging has appeared.

Features

  • Usable in CO₂ incubator
  • Compact microscope
  • Just connect to PC with USB
  • Multiple units can be controlled by one PC
  • Fluorescent imaging (WSL-1800-B CytoWatcher FL)
  • 5M Pixels color digital camera

Description

Usable in CO₂ incubator

Since CytoWatcher body is moisture-proof, it can take time-lapsed images of live cells with cultured in a high humidity CO₂ incubator. There is no need to use an expensive microscope stage culture system.

Multiple units can be controlled by one PC

CytoWatcher is used by connecting it to a Windows PC (option) with a USB 3.0 cable (power is supplied from the PC via USB). If you start the control software ImageSaverT (included as standard), you can see live cell images immediately. You can also save images and take time-lapse images easily.

Multiple CytoWatchers can be connected to one PC and used (up to 4 units) for simultaneous time-lapse imaging of multiple samples in parallel.

5M Pixels color digital camera

CytoWatcher has applied 4x object lens with 5M pixels color CMOS camera.

Field of view: 1.425 mm × 1.070 mm

Fluorescent imaging (WSL-1800-B CytoWatcher FL)

The fluorescent imaging model is equipped with a blue LED light source and an excitation/emission filter and supports green emitted fluorescent imaging such as GFP. It is possible to set it up in a CO₂ incubator and combine it with bright field and fluorescent time-lapse imaging.

Applications

Growth of NIH3T3 cells

Mouse fibroblast NIH3T3 cells on a 10 cm dish, Imaging for 72 h (30 minutes-intervals)

Wound healing assay

Imaging for 24 h (30 minutes-intervals) after scratching the cultured layer of 100% confluent NIH3T3 cells on a 35 mm dish

Differentiation into Adipocyte-like cells

Swiss 3T3 L-1 cells imaging for 7days (1 h-intervals) after replacing to the differentiation inducing medium (DMEM, 10 % FBS, 250 nM Dexamethazone, 0.5 mM Isobutylmethylxanthin, 10 ug/mL Insulin)

IVF bovine embryos

Time-lapse monitoring bovine embryos 6 hours after in vitro fertilization for 9 days (0.5 h-intervals) in an incubator

By courtesy of Kei Imai, Department of Sustainable Agriculture, Rakuno Gakuen University, Japan

GFP fluorescent cell imaging

Fluorescence time-lapse images of NIH 3T3 cells (a 35 mm glass-bottom dish) for 48 h after transfection of the EGFP expression vector, using a fluorescent imaging model of CytoWatcher.

Brochure

Specifications

 WSL-1800 CytoWatcherWSL-1800-B CytoWatcher FL
Camera5M pixels color CMOS camera
Resolution2592 x 1944 pixels
Magnification4 x
Field of view1.425 x 1.070 mm
FocusingManual adjustment
Light sourceWhite LED (trans-illumination)White LED (trans-illumination)
465 nm epi-blue LED (Side-illumination)
Optical filter-Excitation: 480 nm shorpass filter
Emission: 525/45 nm bandpass filter
Moisture resistantUsable in humidity 95% RH (usable in a CO₂ incubator)
Control softwareImageSaverT for Windows (included as standard)
Capture mode: Live / Time-lapse
File format8 bit TIFF, BMP, JPEG, Color / Monochrome
Movie file: avi, maximum 60 fps
PC connectionUSB 3.0 x 1USB 3.0 x 1, USB 2.0 (or 3.0) x 1
OSWindows 10 / 8.1 / 7 (64 / 32 bit)
Dimension130 (W) x 180 (D) x 190 (H) mm130 (W) x 180 (D) x 190 (H) mm
Weight2.5 kg2.7 kg
PowerUSB (bus powered)

Ordering Information

Code No.DescriptionUnit
3601800WSL-1800 CytoWatcher1 set
3601803WSL-1800-B CytoWatcher FL1 set
3601809USB 3.0 extension cable1 ea
2110030CS Analyzer version 4 for Windows1 set
2140202UP-X898MD SONY thermal printer1 set
2140913UPP-110HG printer paper, 10 rolls/pk1 set

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