Purpose and Application
- For bright field cell imaging with low magnification.
- Compact size, no stress to take space.
- Usable in a CO₂ incubator, for time-lapse imaging in culture condition.
- Equipped with 5M pixels color CMOS camera.
- Multiple samples can be taken by connecting multiple units.
- A model for fluorescent imaging has appeared.
- Usable in CO₂ incubator
- Compact microscope
- Just connect to PC with USB
- Multiple units can be controlled by one PC
- Fluorescent imaging (WSL-1800-B CytoWatcher FL)
- 5M Pixels color digital camera
Usable in CO₂ incubator
Since CytoWatcher body is moisture-proof, it can take time-lapsed images of live cells with cultured in a high humidity CO₂ incubator. There is no need to use an expensive microscope stage culture system.
Multiple units can be controlled by one PC
CytoWatcher is used by connecting it to a Windows PC (option) with a USB 3.0 cable (power is supplied from the PC via USB). If you start the control software ImageSaverT (included as standard), you can see live cell images immediately. You can also save images and take time-lapse images easily.
Multiple CytoWatchers can be connected to one PC and used (up to 4 units) for simultaneous time-lapse imaging of multiple samples in parallel.
5M Pixels color digital camera
CytoWatcher has applied 4x object lens with 5M pixels color CMOS camera.
Field of view: 1.425 mm × 1.070 mm
Fluorescent imaging (WSL-1800-B CytoWatcher FL)
The fluorescent imaging model is equipped with a blue LED light source and an excitation/emission filter and supports green emitted fluorescent imaging such as GFP. It is possible to set it up in a CO₂ incubator and combine it with bright field and fluorescent time-lapse imaging.
Growth of NIH3T3 cells
Mouse fibroblast NIH3T3 cells on a 10 cm dish, Imaging for 72 h (30 minutes-intervals)
Wound healing assay
Imaging for 24 h (30 minutes-intervals) after scratching the cultured layer of 100% confluent NIH3T3 cells on a 35 mm dish
Differentiation into Adipocyte-like cells
Swiss 3T3 L-1 cells imaging for 7days (1 h-intervals) after replacing to the differentiation inducing medium (DMEM, 10 % FBS, 250 nM Dexamethazone, 0.5 mM Isobutylmethylxanthin, 10 ug/mL Insulin)
IVF bovine embryos
Time-lapse monitoring bovine embryos 6 hours after in vitro fertilization for 9 days (0.5 h-intervals) in an incubator
By courtesy of Kei Imai, Department of Sustainable Agriculture, Rakuno Gakuen University, Japan
GFP fluorescent cell imaging
Fluorescence time-lapse images of NIH 3T3 cells (a 35 mm glass-bottom dish) for 48 h after transfection of the EGFP expression vector, using a fluorescent imaging model of CytoWatcher.
|WSL-1800 CytoWatcher||WSL-1800-B CytoWatcher FL|
|Camera||5M pixels color CMOS camera|
|Resolution||2592 x 1944 pixels|
|Field of view||1.425 x 1.070 mm|
|Light source||White LED (trans-illumination)||White LED (trans-illumination)
465 nm epi-blue LED (Side-illumination)
|Optical filter||-||Excitation: 480 nm shorpass filter
Emission: 525/45 nm bandpass filter
|Moisture resistant||Usable in humidity 95% RH (usable in a CO₂ incubator)|
|Control software||ImageSaverT for Windows (included as standard)
Capture mode: Live / Time-lapse
|File format||8 bit TIFF, BMP, JPEG, Color / Monochrome
Movie file: avi, maximum 60 fps
|PC connection||USB 3.0 x 1||USB 3.0 x 1, USB 2.0 (or 3.0) x 1|
|OS||Windows 10 / 8.1 / 7 (64 / 32 bit)|
|Dimension||130 (W) x 180 (D) x 190 (H) mm||130 (W) x 180 (D) x 190 (H) mm|
|Weight||2.5 kg||2.7 kg|
|Power||USB (bus powered)|
|3601800||WSL-1800 CytoWatcher||1 set|
|3601803||WSL-1800-B CytoWatcher FL||1 set|
|3601809||USB 3.0 extension cable||1 ea|
|2110030||CS Analyzer version 4 for Windows||1 set|
|2140202||UP-X898MD SONY thermal printer||1 set|
|2140913||UPP-110HG printer paper, 10 rolls/pk||1 set|