WSE-7051 EzRun TBE

Purpose and Application

Buffer for electrophoresis of DNA

Features

Sterilized, DNase free
Suitable for analyzing low molecular weight DNA fragments
Storable for 1 year at room temperature

Tips for Agarose Gel Electrophoresis

The type of buffers used in agarose gel electrophoresis can be determined by the size of the DNA fragment and the content of the post-migration experiment.

If you want to isolate large DNA greater than or equal to 15,000 bp, use the TAE buffer. Use a TBE buffer if you don’t want to extract DNA or don’t care about recovery rates after migration with smaller DNA of 1,000 bp or less.

The above diagram shows the results of an EzDNA Ladder applied to a 1.5% agarose gel using EzRunTAE or EzRunTBE buffers and electrophoresis at 150V for 20 minutes using a Submerge-Mini. As you can see, the TAE buffer is suitable for polymer separation and the TBE buffer is suitable for low molecular separation.

Specifications

	WSE-7051 EzRun TBE
Major components	Tris, Boric acid, EDTA, 10x concentrated solution
Package	500 mL/bottle
Preparation	10x dilute with distilled water
Applicable amount	For 5L, 220 mL per 1 run (About 22 runs with ATTO Submerge Mini chamber)
Storage	For 1 year at room temperature (unopened)