PAGEL

Purpose and Application

Separation and detection of proteins
Second step of 2D electrophoresis
SDS-PAGE, Native-PAGE
Detection of PCR products, separation of DNA
Screening
Experimental training for students or researchers

Features

Mini size (90 mm x 78 mm)
Offers stability and reproducibility of separation
Glass plates with good thermal stability and uniformity employed
Easy to set on to the apparatus and take off the gel from the glass plate
Varieties of gels are available, including wide range of gel percentage
If you purchase more than 1 pk/month, you can rent the AE-6530 mPAGE for free!
Compatible electrophoresis apparatus: ATTO Mini Gel Size Electrophoresis System (WSE-1150P PageRunAce, WSE-1100P PageRun-R, AE-6531P PageRun, AE-6530P mPAGE, AE-6500 Dual Mini Slab, WSE-1165 Mini-Slab)
※This gel cast includes plates whose total thickness is 5 mm, so Plate holder for PAGEL are required.

Data

Pattern data of protein samples (SDS-PAGE)

Gel: SPG-520L (5~20%, 12-well gel)

Sample: M.W. marker etc.

Running buffer: AE-1410 EzRun (25 mM Tris, 192 mM Glycine, 0.1% SDS)

Electrophoresis system: AE-6530 mPAGE

Condition: Constant current 20 mA, 80 min

Staining: AE-1340 EzStain AQua (CBB staining)

Pattern data of Protein samples (Native-PAGE)

Gel: NPG-520L (5~20%, 12-well gel)

Sample: Protein standard substance samples etc.

Running buffer: WSE-7055 EzRunTG (25 mM Tris, 192 mM Glycine)

Electrophoresis system: AE-6530 mPAGE

Condition: Constant current 20 mA, 80 min

Staining: EtBr staining

Specifications

	NPU-5L PAGEL	NPG-310L PAGEL	NPG/SPG-520L PAGEL
Usage	Polyacrylamide gel electrophoresis of protein or nucleic acid (SDS-PAGE, native PAGE etc)
Glass plate size	120 (W) x 100 (H) mm
Gel size	90 (W) x 83 (H) mm, thickness 1 mm
Gel/buffer composition	5% polyacrylamide gel	3~10% Gradient gel	5~20% Gradient gel
Sample wells	12-wells (width 4.5 mm)
Separation range	50~600 kDa 250~4000 bp	20~600 kDa 50~4000 bp	10~400 kDa 20~2000 bp
sample loading volume	maximum 28 μL/well
Storage	6 months when stored in a refrigerator (NOT to be frozen)
Package	10 plates/box

* Gels do NOT contain SDS. Electrophoresis with buffer solution containing 24mM Tris + 192mM Glycine + 0.1% SDS (AE-1410 EzRun) is Laemmli method, and one without SDS (WSE-7055 EzRun TG) is Ornstein-Davis method.

* Use buffer solution containing 25mM Tris and 192mM Glycine or our product, [WSE-7055 EzRunTG] for DNA electrophoresis.

* PAGEL and e-PAGEL have slightly different gel making methods, so the migration patterns are not exactly the same.

Ordering Information

Code No.	Description	Unit
2331192	NPU-5L PAGEL 5% 12-well 10 gels	1 pk
2331194	NPG-310L PAGEL 3~10% 12-well 10 gels	1 pk
2331150	NPG-520L PAGEL 5~20% 12-well 10 gels	1 pk
2331120	SPG-520L PAGEL 5~20% 12-well 10 gels	1 pk